evidence supports the involvement of nitric oxide (no) in a varity of male reproductive processes such as spermatogenesis, spermiogenesis, sperm motion, sperm metabolism and sperm capacitation. however, low concentration of no is essential in biology and physiology of spermatozoa, but high amounts of no is toxic and has negative effects on sperm functions. on the other hand, it is established t...

background: it is established that sperm dna integrity is essential in fertilization and normal embryo and fetal development. routine semen analysis gives an approximate evaluation of the functional competence of spermatozoa, but does not always reflect the quality of sperm dna. therefore, the evaluation of sperm dna integrity, in addition to routine sperm parameters, could add further informat...

this study was conducted to determine chromatin integrity and dna damage by dna electrophoresis and comet assays of buffalo fresh and frozen semen. semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and dna damage. a significant variation was found in semen parameters after thawing. highly sig...

This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

background: when male animals die, spermatozoa within the body of animal will be degenerated. because of unique chromatin structure of sperm, maybe this degeneration is different from other cells. however there is not any research which considered directly the integrity of sperm dna by keeping the cadaver in refrigerator. objective: the aim of this study was to assess viability, total motility ...

Background: Varicocele occurs in approximately 15% to 20% of the male population and it is the most common cause of poor semen parameters. It has been demonstrated that patients with varicocele have poor chromatin packing, DNA damage and nuclear anomalies than the healthy men. Therefore, the aim of this study was to evaluate sperm chromatin integrity and semen parameters in these patients after...

background: sperm selection for icsi based on morphology and motility might not be relevant to chromatin integrity. thus sperm selection based on sperm characteristics has been suggested. objective: the aim of this study was to compare the efficiency of zeta method with routine density gradient centrifugation method (dgc) for the selection of sperm with higher dna integrity. materials and metho...

fertilization involves direct interaction of the sperm and oocyte, fusion of the cell membranes and :union: of male and female gamete genomes. the completion of this process and subsequent embryo development depend in part on the inherent integrity of the sperm dna. sperm genome quality has been emphasized for several years as playing a major role in early embryogenesis. there is clinical evide...

background: there are few studies indicating the detrimental effects of ibuprofen on sperm fertility potential and dna integrity. objective: to determine the effects of ibuprofen on sperm parameters, chromatin condensation and dna integrity of mice. materials and methods: in this experimental study, 36 adult male mice with average weight 37 gr were divided into three groups, including control (...