methods genomic dna of cultured s. agalactiae was prepared and loop-mediated isothermal amplification (lamp) primers were designed based on the camp gene in bacteria. the optimum primer set was selected based on the reaction speed and specificity. the reaction result was monitored visually. the sensitivity and specificity of the lamp method were evaluated and compared with polymerase chain reac...

conclusions species identification of trichophyton is crucially important for epidemiological and phylogenetic purposes and for genotype delineation. rca based on its polymorphisms can be used to generate identification barcodes and as an alternative to dna sequencing; it is a very fast, specific, and economical tool for species identification. results of the 61 putative trichophyton clinical i...

Nickel phthalocyanine complex containing 4-(3,4-dicyanophenoxy)-3-methoxybenzoic acid group was synthesized and specified by way of FT-IR, NMR, UV/Vis procedures. The binding PcNi to CT-DNA examined via electronic absorption titration, emission melting temperature, viscosity measurement, agarose gel electrophoresis technics, respectively. DNA interaction activity against studied UV/Vi titration...

DNA direct amplification from biological samples such as saliva and blood has proven to be successful but can more challenging minute trace DNA.

Sensitive detection of miRNA-21 provides remarkable results for the diagnosis early breast cancer. Here, we fabricated a novel electrochemical biosensor ultrasensitive via in situ reduction gold nanoparticles (AuNPs)-decorated Ti 3 C 2 MXene, combined with cascaded signal amplification strategy, that is, strand displacement DNA walker-induced multi-DNA (product DNA) release and cyclic enzymatic...

The development of 2 laboratory technics for more accurate quantification of hematopoietic activity, (a) radioactive iron (SgFe) uptake and (b) DNA content of marrow cells, are described, utilizing a regenerating marrow system in rabbits after local irradiation of 1 femur while shielding the contralateral leg. The sensitivity and reproducibility of the 2 methods were determined; there was good ...

Background: Amplification of HER2 is seen in 20-30% of breast cancer cases. Measurement of HER2 gene amplification appears to be of vital importance in planning the treatment schedule for patients with breast carcinoma. The aim of our study was to evaluate HER2 amplification status in malignant and benign breast tumors by differential PCR (dPCR). Materials and Methods: The genomic DNA was ex...