background hepatocytes are used as an in vitro model to evaluate drug metabolism. human hepatocyte transplant has been considered as the temporary treatment of acute liver failure. optimization freezing methods is very important to preserve both cell viability and function which are achieved by cryopreservation mostly always. objectives the present study aimed to investigate the cryoprotective ...

We examined effects of heat, zinc, ion, and dithiothreitol in restoring the activity of lead-inhibited-5-aminolevulinate dehydratase (EC 4.2.1.24). The ratio of non-activated to activated activity produced by dithiothreitol correlated well with blood lead concentration among 35 lead workers. The individual effects of heat, zinc, or dithiothreitol differ from each other in the shift of pH optimu...

I studied activation of serum creatine kinase (CK) by sulfhydryl reagents, and the optimum assay conditions.Dithiothreitol and -mercaptoethanol were the best activators. Dithioerythritol, L-cys. teine,glutathione,and mercaptoacetatewere less effective. Over a dithiothreitol concentration range from 0.02 to 2 mmol/liter, enzyme activity increased with increasing dithiothreitol. Activation was ma...

1 Histamine produced dose-dependent contractile responses on both isolated perfused ear arteries and aortic strips of the rabbit. These responses were blocked by mepyramine and potentiated by both metiamide and dithiothreitol. 2 In the presence of maximum potentiation by metiamide, dithiothreitol still potentiated the contractile response to histamine of both preparations. 3 In the presence of ...