The cardiac muscle protein titin, responsible for developing passive elasticity and extensibility of muscle, possesses about 40 immunoglobulin-like (Ig) domains in its I-band region. Atomic force microscopy (AFM) and steered molecular dynamics (SMD) have been successfully combined to investigate the reversible unfolding of individual Ig domains. However, previous SMD studies of titin I-band mod...

The main function purpose of nanobiosensors is to sense a biologically specific material and the kind of sensing platform and doping engineering has been an emerging topic and plays an important role in monolayer molybdenum disulfide (mMoS2). In this paper, we theoretically reveal the electronic structures of mMoS2 doped by 3d transition metals. Furthermore, adsorption of nucleic acid [Adenine ...

The circular dichroic spectra of oxytocin and four analogues of oxytocin are presented at several pH values in the wave length interval, 220 to 320 rnp. The analogues are 2-isoleucineand 2-glycine-oxytocin, in which the tyrosine residues in Position 2 have been replaced by isoleucine and glycine, respectively, as well as deamino-oxytocin and deamino-2-isoleucine-oxytocin, in which the a-NH2 gro...

A simple band model such as the effective mass approximation (EMA) can be used to quickly obtain lower-energy region for structure of monolayer molybdenum disulfide. But EMA cannot give correct description in higher-energy region. To address this major issue, we propose an analytical calculation (ABC) study Important parameters ABC are obtained by fitting three-direction disulfide from first-pr...

The spectral properties of Bowman-Birk soybean trypsin inhibitor (BBI) were investigated by analyzing difference absorption spectra and difference CD spectra and by comparing them with those of tyrosyl model compounds. The O-acetylation of tyrosyl side chains showed that the ultraviolet CD bands of BBI above 225 nm originate from disulfide side chains and tyrosyl phenolic groups; phenylalanyl r...

Human plasma apolipoprotein (apo) D is a glycoprotein with an apparent molecular weight of 29,000 M(r). It is present, mainly, in high density lipoproteins (HDL) and very high density lipoproteins (VHDL). Western blot analysis of HDL and VHDL using rabbit antibodies to human apoD revealed major immunoreactive bands at 29,000 and 38,000 M(r), with minor bands ranging from 50,000 to and 80,000 M(...

The spectral properties of tyrosyl side chains were investigated in chicken ovomucoid and derivatives produced by acetylating with N-acetylimidazole. Cooling ovomucoid to 77°K brings out the tyrosyl fine structure. Absorption spectra at 77°K reveal 2 different kinds of tyrosyl sites. About 4 tyrosyls have their longest wavelength absorption band at 284 nm and are relatively well shielded from t...

The compound di-N-(2-nitro-4-azidophenyl)cystamineS,S-dioxide (DNCO) has been synthesized and characterized. In a dark reaction this reagent will undergo efficient disulfide-sulfhydryl exchange with protein -SH groups, thus providing a covalently bound photoactivatable group. The cross-linked products obtained in applying this reagent to the soluble protein rabbit muscle aldolase have pointed u...