conventional methods of pear breeding, largely based on intra- and inter-specific hybridization, are difficult because pear is highly heterozygous, polygenic and has a long juvenile period. genetic improvements of pear cultivars are possible through induction of mutations and gene transfer by genetic engineering. a general prerequisite for these approaches is to establish an efficient plant reg...

Conventional methods of pear breeding, largely based on intra- and inter-specific hybridization, are difficult because pear is highly heterozygous, polygenic and has a long juvenile period. Genetic improvements of pear cultivars are possible through induction of mutations and gene transfer by genetic engineering. A general prerequisite for these approaches is to establish an efficient plant reg...

In order to select the best tomato cultivar for Agrobacterium mediated gene transformation studies, In vitro regeneration frequency of hypocotyls, leaf disc and shoot tip of five tomato cultivars (Lycopersicon esculentum) was investigated on a regeneration medium supplemented with 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. Significant differences in regeneration capacity between genotypes...

This experiment was performed to optimize the medium for tissue culture of Taraxacum kok-saghyz Rodin. Different tissue culture approaches such as shoot regeneration from seed, callus formation from leaf explants and plant regeneration from callus were investigated in this study. All the explants were cultured on MS basal medium supplemented with 20g/l sucrose, 7g/l agar and different plant gro...

A procedure was developed for in vitro propagation of Curculigo latifolia through shoot tip culture. Direct regeneration and indirect scalp induction of Curculigo latifolia were obtained from shoot tip grown on MS medium supplemented with different concentrations and combinations of thidiazuron and indole-3-butyric acid. Maximum response for direct regeneration in terms of percentage of explant...

Cotton (Gossypium spp.) belonging to the genus Gossypium in the family Malvaceae. Cotton is an important fibre crop of global importance. It is grown in tropical and subtropical regions of more than 80 countries with an annual production of 20 million tonnes. It is an important source of oil and high quality protein meal and plays a significant role in the national economy. Among the cotton pro...

An efficient and reproducible procedure was established for direct shoot regeneration of Solanum surattense Bum. using internodal explants. The shoot regeneration efficiency on Murashige and Skoog (MS) medium supplemented with 6benzylaminopurine (BAP) was superior in compared to 6furfurylaminopurine (Kn). The highest mean number of shoots per explant was recorded on MS medium containing 0.5 mgl...

abstract lettuce (lactuca sativa l.) is a major leafy vegetable and belongs to the asteraceae family (compositae). the genetic manipulation of lettuce requires a reliable and efficient regeneration method. since lettuce is a suitable bioreactor for production of recombinant proteins especially edible vaccines. this study was conducted to identify an appropriate method for lettuce gene transform...

abstract in vitro leaf explants of gf677 hybrid rootstock and rabi cultivar were placed on mql medium supplemented with bap at 0, 1, 2, 3 and 4 mg/l and naa at 0, 0.15 and 0.3 mg/l concentrations. the results revealed that bap, naa, genotype and their interactions had significant effect on both percentage and mean number of the regenerated shoots. for gf677, the highest percentage of shoot rege...