Keynan, A. (University of Wisconsin, Madison), W. G. Murrell, and H. O. Halvorson. Germination properties of spores with low dipicolinic acid content. J. Bacteriol. 83:395-399. 1962.-When the dipicolinic acid content of spores of Bacillus cereus strain T is reduced from 7.5 to 2 or 3%, the spores germinate spontaneously after heat activation and are sluggish in their response to l-alanine and o...

Conditions under which dipicolinic acid may be released from bacterial spores probably relate to the chemical state of the dipicolinic acid in the spore, to its physical location in the spore, and to its influence on the physiological properties of the spore. The latter aspect embraces the relation between release of dipicolinic acid and the viability of the spore, its heat resistance, and its ...

The discovery and certain aspects of the biosynthesis of dipicolinic acid (pyridine-2,6-dicarboxylic acid) in exudates of germinated spores and in intact spores of aerobic bacilli have been recently reported (Powell, 1953; Perry and Foster, 1955). In the latter paper it was noted that maximum recovery of dipicolinic acid from intact spores necessitated an initial treatment with hot dilute inorg...

A gas-liquid chromatographic procedure has been developed to quantitate dipicolinic acid in bacterial spores. The culture, washed from a plate, was hydrolyzed with acid containing the internal standard, pyridine-2,4-dicarboxylate, and then extracted into methyl isobutyl ketone. The internal standard and dipicolinic acid were then extracted into a small volume of trimethylphenylammonium hydroxid...

background and objectives: microbial spoilage of juices and industries related with alicyclobacillus are considerable international issues. this spore-forming bacterium causes changes in juices odor and taste. the isolation and identification of alicyclobacillus contamination in juice producing and packaging industries has an essential role in the prevention and control of this type of spoilage...

Beta-attenuation analysis of intact spores of Bacillus megaterium containing tritium-labeled dipicolinic acid has shown that dipicolinic acid is located in the spore protoplast and not in the cortex.

Spores of Bacillus cereus T lacking dipicolinic acid showed a statistically significant reduction in resistance to ultraviolet and gamma radiation as compared with spores with high dipicolinic acid content.

Very little is known of the precursors of the dipicolinic acid (pyridine-2,6-dicarboxylic acid) in bacterial spores. C'4-2,6-diaminopimelic acid was shown to contribute to the formation of dipicolinic acid in Bacillus cereus var. mycoides (Perry and Foster, 1955; Foster, 1956) but the efficiency of the conversion was low, possibly because of a slow penetration of the cells by the exogenously su...

Keynan, A. (University of Wisconsin, Madison) and H. O. Halvorson. Calcium dipicolinic acid-induced germination of Bacillus cereus spores. J. Bacteriol. 83:100-105. 1962.-The germination of spores of Bacillus cereus strain T can be initiated by calcium dipicolinic acid. The kinetics of germination are characterized by a long lag period followed by a rapid loss of refractility. The lag period di...

s: It was found that when dipicolinic acid is added to the culture solution of Bacillus subtilis natto, the area of fibrin dissolved by nattokinase (standard fibrin plate) and the amidase activity of nattokinase against Suc-Ala-Ala-Pro-PhepNA increased. For example, when manufacturing natto using steamed soybeans, the addition of 10-64 mM of dipicolinic acid increases amidase activity by more t...