introduction : albumin, one of the most important plasma proteins, has a difficult process of synthesis and production. we compared two different methods for albumin purification: carboxymethyl cellulose (cm cellulose) resin exchange and diethylaminoethyl cellulose (deae cellulose) resin exchange in order to determine which resin could be more beneficial. materials and methods : two ion exchang...

A toxin that causes morphological changes in Chinese hamster ovary cells was isolated from a culture filtrate of Shigella dysenteriae 1. It was partially purified by successive column chromatography on diethylaminoethyl-cellulose, Bio-Gel A-5m, and hydroxylapatite. The toxin was separated from neurotoxin by diethylaminoethyl-cellulose column chromatography and was not lethal for mice.

Subcellular fractions from the cotyledon obtained by differential and density gradient centrifugation, and extracts of total proteins from both cotyledon and axial tissues were analyzed by diethylaminoethyl cellulose chromatography, zone electrophoresis, ultracentrifugation, immunodiffusion, and immunoelectrophoresis. Fractionation and characterization of proteins in subcellular organelles of t...

A novel anion-exchange column for fast-flow liquid chromatography was developed. The column was prepared by immobilizing diethylaminoethyl groups on to the inner walls of regenerated cellulose hollow fibres. A detailed investigation was conducted of the parameters affecting diethylaminoethyl immobilization such as the NaOH concentration, the reaction temperature, the diethylaminoethyl concentra...

Since the initial description (2, 3) of procedures for the use of cellulose ion exchange columns for the chromatography of proteins, a number of such systems have been developed for specific proteins, including the heme proteins. Thus, Gutter et al. (4) have described the use of carboxymethyl cellulose in a study of various hemoglobins, Rumen (5) has utilized carboxymethyl cellulose for the ide...

Thyroglobulin may be defined as the iodine-containing protein of the thyroid gland which has a sedimentation coefficient of 4o.w = 19 S, an electrophoretic mobility at pH 8.6 of approximately 5 x 10F5 cm2 volt-l set-1, and a steep salting out curve at about 38% ammonium sulfate saturation at neutral pH (1). Although other iodoproteins, both soluble in neutral salt and attached to cell particles...

Molecular heterogeneity of estrogen receptors in cytosols from human breast carcinomas was demonstrated with regard to size and surface ionic charge. Following separation of the molecular forms of estrogen receptors into either the 8S or the 4S species by sedimentation on linear gradients of sucrose, diethylaminoethyl cellulose chromatography was used to fractionate the various com ponents in t...

Molecular heterogeneity of estrogen receptors in cytosols from human breast carcinomas was demonstrated with regard to size and surface ionic charge. Following separation of the molecular forms of estrogen receptors into either the 8S or the 4S species by sedimentation on linear gradients of sucrose, diethylaminoethyl cellulose chromatography was used to fractionate the various com ponents in t...