A fixed microelectrode device for cell stimulation has been designed and fabricated using micro-electro-mechanical systems (MEMS) technology. Dielectrophoretic forces obtained from non-uniform electric fields were used for manipulating and positioning osteoblasts. The experiments show that the osteoblasts experience positive dielectrophoresis (p-DEP) when suspended in iso-osmotic culture medium...

Dielectrophoresis is a noninvasive, nondestructive, inexpensive, and fast technique for the manipulation of bioparticles. Recent advances in the field of dielectrophoresis (DEP) have resulted in new approaches for characterizing the behavior of particles and cells using direct current (DC) electric fields. In such approaches, spatial nonuniformities are created in the channel by embedding insul...

Dielectrophoresis-assisted growth of metallic nanowires from an aqueous salt solution has been previously reported, but so far there has been no clear understanding of the process leading to such a bottom-up assembly. The present work, based on a series of experiments to grow metallic nano- and microwires by dielectrophoresis, provides a general theoretical description of the growth of such wir...

The authors present dielectrophoresis DEP -based tweezers that can be used to characterize the interactions between a particle and the surface it is attached to, within a microfluidic device. As a proof of concept, 5.4 m polystyrene beads functionalized by carboxyl group were attached on a bare and poly-L-lysine functionalized oxide surface. Negative dielectrophoresis force was generated using ...

Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresi...

In this study, the dielectrophoretic response of prostate tumor initiating cells (TICs) was investigated in a microfluidic system utilizing contactless dielectrophoresis (cDEP). The dielectrophoretic response of prostate TICs was observed to be distinctively different than that for non-TICs, enabling them to be sorted using cDEP. Culturing the sorted TICs generated spheroids, indicating that th...

---A fixed microelectrode device for cell stimulation has been designed and fabricated using microelectro-mechanical systems (MEMS) technology. The dielectrophoretic forces obtained from fixed electrode structures producing non-uniform electric fields were then used for manipulating and positioning obsteoblast cells. Our experiments show that the osteoblasts experience positive dielectrophoresi...

This article presents use of electric field for manipulation of biological cells and modification of their physical properties in microfluidic chambers. Some fabrication procedures of electrodes and microfluidic chambers are presented, from thin film metal electrodes to three-dimensional structures. Strong electric field pulses induce formation of pores in cell membrane (electroporation), which...