It is often desirable to obtain gene libraries with the greatest possible number of variants. We tested two different methods for desalting the products of library ligation reactions (silica-based microcolumns and drop dialysis), and examined their effects on final library size. For both intramolecular and intermolecular ligation, desalting by drop dialysis yielded approximately 3-5 times more ...

Desalting/dehydration plants (DDP) are often installed in crude oil production units in order to remove water-soluble salts from an oil stream. This paper describes the development of simple inferential estimators for product quality of the desalting/dehydration process. The inferential estimators were constructed to capture the relationship between the product quality of the plant and the proc...

..................................................................................................... 40 Introduction................................................................................................. 41 Experimental section....................................................................................... 46 Materials..............................................................

The ability to control the ionic environment in saline waters and aqueous electrolytes is useful for desalination as well as electronic biosensing. We demonstrate a method of electronic desalting at micro-scale through on-chip micro electrodes. We show that, while desalting is limited in bulk solutions with unlimited availability of salts, significant desalting of ≥1 mM solutions can be achieve...

Continue survival of the petrochemical industry in the face of advancement in processing technology must change for better performance. In doing so, the pre-treatment stage of crude, which include desalting will go a long way at improving the overall quality of the product obtained. It is in view of this that a mathematical modeling of adsorption of a fixed simulated bed for desalting operation...

► Self-adaptive mode of feed-flow reversal (FFR) was developed for RO desalting. ► FFR was triggered via mineral scale detection using an external membrane

Oligonucleotides synthesized on a montmorillonite catalyst were analyzed directly. By mixing the catalyst with a matrix (2,4,6-trihydroxyacetophenone or 6-aza-2-thiothymine) and dibasic ammonium citrate, higher molecular weight products were detected compared with "classical" methods such as gel electrophoresis and HPLC with UV as a detector. The oligomers (30-mers and higher) were detected by ...

The adverse effects of nonvolatile salts on the electrospray (ESI) mass spectra of proteins and other biological analytes are a major obstacle for a wide range of applications. Numerous sample cleanup approaches have been devised to facilitate ESI-MS analyses. Recently developed microdialysis techniques can shorten desalting times down to several minutes, the bottleneck being diffusion of the c...