Salmonella typhimurium was found to utilize 2-deoxyribose as a sole carbon and energy source. Cells grown in the presence of deoxyribose contained increased levels of deoxyribose kinase, thymidine phosphorylase, and two forms of deoxyribose-5-phosphate aldolase (DR5P aldolase). One form of DR5P aldolase was induced by deoxyribose and coordinately regulated with deoxyribose kinase. The second fo...

A phosphodeoxyribomutase has been demonstrated in cell-free extracts of Sarcina lutea. The enzyme could be heated to 55 ° for at least 2 h and still retain activity. It had a pH optimum around 7.2, and the most highly purified preparation showed a specific activity of approx. 60 ~moles of deoxyribose 1-phosphate converted to deoxyribose 5-phosphate/mg protein/h. The enzyme was constitutive in t...

The fermentation of deoxyribose in Escherichia coli (1, 2) and in Lactobacillus plantarum (3, 4) appears to involve 2-deoxyribose 5-phosphate as an intermediate. Racker (2) has described an enzyme, deoxyribose phosphate aldolase, which catal.yzes the reversible cleavage of deoxyribose 5-phosphate to acetaldehyde and glyceraldehyde 3-phosphate. The enzyme occurs in E. coli and in animal tissues ...

Phosphodeoxyribomutase, the enzyme which catalyzes the interconversion of 2-deoxyribose-1-phosphate to 2-deoxyribose-5-phosphate, has been partially purified from Salmonella typhimurium. The enzyme had an absolute requirement for manganese ion and was stimulated by glucose-1, 6-diphosphate. Phosphodeoxyribomutase was induced by deoxyribose-5-phosphate and was coordinately regulated with the enz...

Lanning and Cohen (1) reported that when Escherichia coli strain B is infected with T6r+ bacteriophage in a medium containing glucose-1-Cr4, the deoxyribose from the deoxyribonucleic acid of the T6rf particles liberated on lysis of the cells contains 40 to 60 per cent of the specific activity of the exogenous glucose. In parallel experiments, it was shown that the deoxyribose from the deoxyribo...

We apply first-principles computational methods to study elastic scattering of low-energy electrons by 2-deoxyribose and 2-deoxyribose monophosphate, which are of interest as components of the DNA backbone, and to tetrahydrofuran (THF), which has been studied as a deoxyribose analog. To investigate the dependence of the scattering process on the molecular conformation, we examine Cs and C2 conf...

The mechanism of the biosynthesis of deoxyribose has been one of the particularly obscure problems in the biochemistry of the nucleic acids. During the past decade, considerable effort was expended in the demonstration that the deoxyribose of deoxyribonucleic acid was not derived via the condensation of glyceraldehyde a-phosphate and acetaldehyde in the presence of deoxyribose phosphate aldolss...

The present study was intended to provide information on the carbon sources of deoxyribose in animal tissues. Some tracer studies (2-5) have indicated that ribose of certain nucleosides or nucleotides is converted to deoxyribose without rupture of the nucleosidic linkage. However, evidence from cell-free preparations of animal tissues (6, 7) as well as microorganisms (6) has suggested an origin...

Deoxyribose test has been widely used for determination of reactivities of various compounds for the hydroxyl radical. The test is based on the formation of hydroxyl radical by Fe2+ complex in the Fenton reaction. We propose a modification of the deoxyribose test to detect strong iron binding, inhibiting participation of Fe2+ in the Fenton reaction, on the basis of examination of concentration ...

Iron chelating agents are essential for treating iron overload in diseases such as beta-thalassemia and are potentially useful for therapy in non-iron overload conditions, including free radical mediated tissue injury. Deferoxamine (DFO), the only drug available for iron chelation therapy, has a number of disadvantages (e.g., lack of intestinal absorption and high cost). The tridentate chelator...