objective dot blot (db) assay provides highly specific results, but usually not reliable for quantification of antibody production. the need for a more objective db assay to provide a better definition of the immune status, against hiv antigens, promoted this study to be done to develop a quantitative db assay. material and methods dot blot (db) strips for antibodies directed to human immunodef...

Metronidazole, ornidazole, tinidazole, and secnidazole are 5-nitroimidazoles. The purpose of this work was to propose a new economical TLC–densitometric method evaluate the chemical stability metronidazole, secnidazole, tinidazole under stress conditions. A forced degradation study performed on silica gel aqueous solutions at various pH values; were prepared in saline hydrogen peroxide, respect...

To establish comprehensive criteria for detecting restenosis and remodeling, inter- and intraobserver reproducibility of quantitative arteriography in the analysis of 20 lesions immediately after and 6 months after percutaneous transluminal coronary angioplasty (PTCA) were assessed. Geometric single-plane (minimum, maximum, mean diameter and percent diameter stenosis), biplane (absolute and rel...

End-stage renal failure (ESRF) is the ultimate consequence of chronic renal failure, and in such cases dialysis is generally required. Almost all dialysed patients have abnormal bone histology and lower values of glomerular filtration rate have been associated with lower bone mineral density (BMD) at all sites. The objective of the present study was to investigate the effect of hemodialysis (HD...

A TLC method with densitometric detection for identification and quantitation of indole compounds in methanolic extracts of fruiting bodies of Armillaria mellea (Vahl.) P. Kumm. (Basidiomycota)--Honey mushroom has been developed. The TLC method with densitometric detection was validated for determination of tryptamine and serotonin contents. The use of this chromatographic-densitometric method ...

The characteristics of computer-assisted imaging devices (CIDs) incorporating charge-coupled device cameras, vidicon cameras and scanning microdensitometers (SMDs) are compared. Advantages of vidicons include their low cost, the option of real-time imaging, continuously variable optical magnification and the wide choice of available vidicon elements. Disadvantages include densitometric non-line...

Atrocapus lakoocha Roxb. or Ma-haad, is a tree in Moraceae family. The main stilbenoid in the heartwood of this plant, oxyresveratrol is an active substance that promotes strong tyrosinase inhibitory and antioxidative effects. In this study, thin layer chromatographic (TLC) – densitometric method was developed and validated to quantitative analyze of oxyresveratrol contents in aqueous and ethan...

DNA analysis was assessed by densitometry for 281 cases of colorectal adenocarcinoma. Detection of aneuploidy in a single case rose from 65% if one, to 92.5% when three or more sections, were analysed. Although aneuploid tumours had significantly larger nuclear areas than near diploid tumours (p = 0.009), densitometric measurements showed no association with clinicopathological variables. DNA c...

Method. A group of 30 healthy trabecular bone specimens were selected to validate the densitometric calibration protocol. Specimens were ashed in a muffle furnace at 650 degrees for 24 hours. The ash density (ρASH) was calculated as the ash weight divided by the specimen volume and was found to range between 200 and 500 mg/cm. The ρASH is considered the golden standard for densitometric analysi...