The quality control involved utilising various methods of frozenram semen and optimising the cryopreservation technology, which are both very important tasks. The experiments were carried out using light microscopy and CTC fluorescent staining on the various stages of ram semen samples preparing for cryopreservation procedure, thawing and incubating (at 39 oC, 2 hours) in the various compositio...

Studies using genetic and biochemical probes have suggested that mouse sperm surface galactosyltransferases may participate during fertilization by binding N- acetylglucosamine (GlcNAc) residues in the egg zona pellucida. In light of these results, we examined sperm surface galactosyltransferase activity during in vitro capacitation to determine whether changes in enzymatic activity correlated ...

The existence of a trypsin-like enzyme (TLE) in acrosomes ofepididymal spermatozoa was confirmed and was further demonstrated to be present in acrosomes of ejaculated and capacitated spermatozoa. TLE rapidly removes the zona pellucida of the ovum. Extracts of acrosomes of ejaculated spermatozoa contain an inhibitor that is separated from the TLE by purification of the TLE. The inhibitor of TLE ...

In 1957, Chang reported the presence of a substance in the seminal plasma of several mammalian species which had the ability to reverse the capacitation process (decapacitation factor or DF). Bedford & Chang (1962) were partially able to purify DF by ultra-centrifugation. Since that time, a great deal of effort has been given to the purification, isolation and identification of this substance (...