Lupin seeds are rich in proteins, lipids and bioactive compounds, but before consumption must be debittered to remove toxic alkaloids. Traditionally, debittering is a water-intensive protracted process, lasting up six days. To develop more efficient procedure, different washing solutions (0.5% 1% NaCl or citric acid), with without the use of ultrasound, were applied on Lupinus albus compared tw...

The observation that the bitterest peptides from casein hydrolysates contain several proline residues led us to hypothesize that a proline-specific protease would be instrumental in debittering such peptides. To identify the desired proline-specific activity, a microbiological screening was carried out in which the chromogenic peptide benzyloxycarbonyl-glycine-proline-p-nitroanilide (Z-Gly-Pro-...

Soya beans and several other beans and cereals have been used as substrates for tempe fermentation with the fungus Rhizopus oligosporus Saito. Except for the presence of alkaloids, the chemical composition of lupins (Lupinus mutabilis Sweet) is similar to that of soya beans. Therefore the potential of lupins for tempe production in regions with a long tradition of lupin consumption is promising...

The levels of quinolizidine alkaloids in lupin seeds vary depending on the botanical and geographical origin of the lupin variety from which theyderive. “Bitter lupins” produce seeds which have a bitter taste due to the higher levels of quinolizidine alkaloids they contain. Bitter lupin seeds are not suitable for human consumption without appropriate pre-treatment (“debittering”). Lupin varieti...

The products of protein hydrolysis, which are normally mixtures of peptides and amino acids, are called protein hydrolysates. Food protein hydrolysates have a wide range of nutritional applications. The production and use of protein hydrolysates differ in the type, level of hydrolysis and the peptide profile. Bitterness in protein hydrolysates is associated with the release of peptides containi...

The technological characteristics of five oleuropeinolytic strains of the Lactobacillus plantarum group selected within 135 isolates from table olives were investigated. The metabolism of phenolic compounds during elaboration of green (cv. Chalkidikis) and black (cv. Kalamata) olives under reduced salt conditions was evaluated. Olives of both cultivars were fermented in two different kinds of b...

This review discusses the debittering enzyme naringinase and its essential role in the commercial processing of citrus fruit juice. Applications of this enzyme in other areas are identified. Characterization of the enzyme is detailed and its immobilized preparations are discussed. Production of microbial naringinase by fermentation is described. © 2000 Elsevier Science Inc. All rights reserved.

The reduction of bitterness in citrus juices would increase their acceptance by the consumer. This reduction in grapefruit juices can be achieved as a result of an enzymatic process, with improved commercial value and maintenance of health properties. The use of a cheap, simple and effective immobilisation method combined with high pressure can be a key asset in the debittering of citrus juices...