نتایج جستجو برای: de gels

تعداد نتایج: 1553782  

2006
Suzanne G. Bolder Hanneke Hendrickx Leonard M.C. Sagis Erik van der Linden

In this paper we describe the rheological behaviour of Ca2+-induced cold-set gels of whey protein mixtures. Coldset gels are important applications for products with a low thermal stability. In previous work [1], we determined the state diagram for whey protein mixtures that were heated for 10 h at pH 2 at 80°C. Under these conditions, the major whey protein, b-lactoglobulin (b-lg), forms fibri...

Journal: :Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie 1987
A T Endler D S Young T Yanagihara R M Currie J Reid

The protein pattern of cerebrospinal fluid (CSF) of 334 patients with various neurological and systemic diseases was investigated by high resolution two-dimensional electrophoresis (2-DE). 2-DE gels of normal CSF contain proteins which are not detectable in 2-DE gels of serum. Disturbances of the blood-brain or blood-CSF barrier, and degenerative diseases of the brain and malignant diseases pro...

2014
Giovanni Modesto Vieira

OBJECTIVE The aim of this study is to determine a protocol of gingival crevicular fluid protein extraction used for the first dimension of 2-DE gels. It also aims at conducting a review on the current candidates for protein markers of this pathology, all of which may be used to prevent the disease. METHODS Gingival crevicular fluid was collected from two groups of 60 patients each, with and w...

Journal: :Cancer research 1980
P K Chan A Feyerabend R K Busch H Busch

The present study was designed to characterize the human tumor nucleolar antigens found first in the HeLa cells and subsequently in a broad range of human cancers. For visualization of the antigens, HeLa cell nucleolar or nuclear protein fractions were analyzed on 4% polyacrylamide isoelectric focusing gels. The gels were incubated with rabbit antisera to HeLa cell nucleoli and then with fluore...

2012
Renato Millioni Rita Polati Michele Menini Lucia Puricelli Manuela Miuzzo Paolo Tessari Enrico Novelli Pier Giorgio Righetti Daniela Cecconi

The performance of two-dimensional electrophoresis in conventional gels in Cartesian coordinates (2-DE) vs. polar coordinates (2-PE) is here evaluated. Although 2-DE is performed in much longer Immobiline gels in the first dimension (17 cm) vs. barely 7-cm in 2-PE, an equivalent resolving power is found. Moreover, due to the possibility of running up to seven Immobiline strips in the radial gel...

Journal: :International journal of oncology 2007
Feng Li Zhiqiang Xiao Pengfei Zhang Jianling Li Maoyu Li Xueping Feng Yongjun Guan Zhuchu Chen

In order to conduct a comparative proteomics study of human nasopharyngeal carcinoma (NPC) to understand the molecular mechanisms that participate in the formation of NPC, the two-dimensional gel electrophoresis (2-DE) reference map of human NPC tissue proteome was described. To provide a high level of reproducibility between gels and accurately array each protein expressed in NPC tissue proteo...

2012
Valentina PESCHETOLA François GALLET Francesco MOLLICA Davide AMBROSI Alain Duperray

The migration process is a physically integrated molecular phenomena that contributes to many physiological motility in vivo processes such as development, immune surveillance and cancer metastasis. To understand cell migration it is necessary to consider the cell’s environment, cell type and morphology as well as the internal organization of the cells, i.e. its cytoskeleton and focal adhesions...

2012
Rosilene Oliveira Mesquita Eduardo de Almeida Soares Everaldo Gonçalves de Barros Marcelo Ehlers Loureiro

The most critical step in any proteomic study is protein extraction and sample preparation. Better solubilization increases the separation and resolution of gels, allowing identification of a higher number of proteins and more accurate quantitation of differences in gene expression. Despite the existence of published results for the optimization of proteomic analyses of soybean seeds, no compar...

A. Farhadyar M. Zare-Dehnavi N. Farhadyar Sh. Aghabeygi,

Nanocomposite of ZrO2/ZnO was prepared under ultrasonic irradiation by sol gel process from directly mixing Zirconium and Zinc gels, and the mixture was placed under ultrasonic irradiation for 2 hours then aging time the filtrated composite gel was calcinated at 500°C for 3h in furnace. The precursor sol of zirconium was prepared from an aqueous solution of ZrCl4 and zinc acetate dihydrated was...

A. Farhadyar M. Zare-Dehnavi N. Farhadyar Sh. Aghabeygi,

Nanocomposite of ZrO2/ZnO was prepared under ultrasonic irradiation by sol gel process from directly mixing Zirconium and Zinc gels, and the mixture was placed under ultrasonic irradiation for 2 hours then aging time the filtrated composite gel was calcinated at 500°C for 3h in furnace. The precursor sol of zirconium was prepared from an aqueous solution of ZrCl4 and zinc acetate dihydrated was...

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