Background: Dirofilaria immitis is a cosmopolitan zoonotic, vector-borne parasite of carnivorous animals causing dirofilariasis in human beings. Common commercial serodiagnostic tests for canine dirofilariasis usually lead to different results in their sensitivity and specificity. The present study reports development of recombinant DgK (rDgK) antigen of D. immitis for accurate immunodiagnosis o...

methoxy poly(ethylene glycol) (mpeg) with molecular mass of 5 kda activated with succinimidyl carbonate and cyanuric chloride, separately was covalently attached to human red blood cells (rbcs). inhibition of agglutination by blood-type specific antisera (anti-d) was employed to evaluate the effect of the polymer coating. the remaining single cells after incubation with anti-d sera were counted...

background: multiple sclerosis (ms) is an autoimmune disease of central nerves system, in which neurological disabilities occur in young adults. despite increasing number of studies on ms, some aspects of this disorder are still unclear. in the previous studies, it has been proven that there is direct relation between ms incidence and vitamin d deficiency. thereby, strong evidence in ms pathoge...

We observed an unexpectedly high incidence of the D-variant placenta! phenotype in Regan isoenzyme-positive sera of cancer patients-. Such D-variant-positive sera exhibit a high degree of inhibition by L-leucine, a phenomenon earlier associated with the Nagao isoenzyme. Unlike normal pla centae, a diffuse phenotype of heat-stable alkaline phosphatase is usually noted in cancer sera containing N...

This study was performed to investigate whethere there is cross-reactivity between Dirofilaria immitis and three intestinal nematodes of dogs. In ELISA, D. immitis-infected dog sera obtained at the 4th molting stage (9-11 weeks) and microfilaremic stage (25-30 weeks) were shown to be highly reactive with crude extract of T. canis. In immunoblotting, some antigenic fractions, 44, 57, 88, 100 kDa...

AIMS To evaluate a rapid immunoblotting procedure for providing evidence of infection with Salmonella typhi using 73 sera from patients infected with S typhi. METHODS A sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE)/immunoblotting procedure using lipopolysaccharide (LPS, O = 9,12) and flagellar (H = d) antigens was used. RESULTS Seventy two of 73 sera contained antibod...

Escherichia coli serA-encoded 3-phosphoglycerate (3PG) dehydrogenase catalyzes the first step of the major phosphorylated pathway of L-serine (Ser) biosynthesis. The SerA enzyme is evolutionarily related to the pdxB gene product, 4-phosphoerythronate dehydrogenase, which catalyzes the second step in one branch of pyridoxal 5'-phosphate coenzyme biosynthesis. Both the Ser and pyridoxal 5'-phosph...

b a ckground: west nile virus (wnv) is a mosquito-borne disease that can cause fatal infection in mammals in-cluding humans, dogs, horses, birds and reptiles. although west nile virus is an asymptomatic infection, especially it can cause neurologic disorders in humans and horses. the aim of this study was to the investigate virological pres- ence of wnv in horses in the black sea region of turk...

Type XVII collagen (BP180), promotes adhesion of basal keratinocytes to epidermal basement membrane, and is the target of disease in patients with certain inherited or acquired blistering diseases. Therefore, the aim of the present study was to investigate IgA autoantibodies using recombinant BP 180 proteins against bullous pemphigoid in the sera of patients. Thirty consecutive BP sera were ana...

Antisera prepared in rabbits by immunization with p-aminophenyl beta-kojibioside conjugated to bovine serum albumin (antikojibiose sera), readily agglutinated whole cells of Streptococcus faecalis or Streptococcus faecium, and showed specific reactions with the lipoteichoic acids (LTAs) of these streptococci by passive hemagglutination, microscale enzyme-linked immunosorbent assay, and crossed ...