Explanted cotyledons from cineraria ‘Jester Pink’ seeds were cultured in vitro on a modified Murashige–Skoog medium containing (in M): 13.5 NAA, 13.5 2,4-D, 4.4 BAP, 13.5 NAA + 4.4 BAP, 13.5 2,4-D + 4.4 BAP or no plant growth regulators (control). Photographs were taken at 7-day intervals for 35 days to follow the embryogenic/organogenic development of the explanted cotyledons. Calorespirometri...

1. Asparagus racemosus is an herb used as a rasayana in Ayurveda. The root of A racemosus is popular for its various medicinal uses such as appetizer, stomachic, tonic aphrodisiac galactogogus etc. In this experiment we have found the shoot induction after 3 weeks when Nodal explants segments were cultured in basal medium supplemented with BAP, Kin and Ads and Shoots induction on MS basal mediu...

A study of callus induction of Tecomella undulata was conducted using a various concentrations of PGRs namely cytokinins such as BAP and KIN and auxins such as 2, 4-D, NAA and IAA. Internodal explants were aseptically cultured on MS medium supplemented with different concentrations (1.03.0 mg/l) of BAP, KIN, 2, 4-D, IAA and NAA. After two weeks callus was formed at the cut surface of the explan...

Purwaceng (Pimpinella alpina) is an herbal plant that has aphrodisiac, diuretic, and tonic properties. The imbalance between the growth exploitation of this makes it included in Appendix I (endangered), so its conservation will be crucial. One method tissue culture. This considered one way to induct callus. research objective find effect various treatments regulator concentrations NAA. 2,4 D co...

Efficient in vitro plantlet regeneration is an important step to successfully transform genes for the improvement of agronomic traits. A combination 6-benzylaminopurine (BAP) and thidiazuron (TDZ) plant growth regulators was applied evaluate shoot capacity whereas α-naphthalene acetic acid (NAA) with (BAP), 2, 4-dichlorophenoxyacetic (2, 4-D) were tested optimize root induction two peanut culti...

An efficient In vitro protocol was established for callogenesis from various plant parts (Leaf, stem, flower bud and tendril) of Passiflora vitifolia Kunth. On Murashige Skoog (MS) basal medium supplemented with concentration auxins (NAA, IAA, 2,4-D) cytokinins (BAP Kn ). Different concentrations (0.5 to 4 mg/L), IAA 2mg/L), NAA 2 mg/l) cytokinin BAP mg/L) were assessed callus induction. Growth...

Chrysanthemum nodal explants were cultured on 1⁄2 X MS medium containing different combinations of growth hormones. A reasonable callus formed on explants when MS contained 0.5 mg. cm each of BAP and NAA after about 3 weeks. Similar results were obtained on MS + 0.5 mg. cm BAP + 0.1 mg. cm NAA or 1.0 mg. cm BAP + 0.1 mg. cm NAA. A maroonish embryogenic callus was formed on MS containing BAP onl...