the activity of aspartate aminotransferase (ast) in human serum has been widely determined as a diagnostic aid in liver disease. in this study, the effect of aluminium on ast isoenzymes in relation to aluminium intoxified patients has been investigated. using gel filtration chromatography technique with sephacryl s-300, mitochondrial aminotransferase (m-ast) and cytosolic aminotransferase (c-as...

Cytosolic tail motifs in host cell surface receptors predict modes of SARS-CoV-2 entry and propagation.

background: to evaluate the effect of cyclosporin a (csa) and angiotensin ii (ang ii) on cytosolic calcium levels in cultured human gingival fibroblasts (hgfs). materials and methods: healthy gingival samples from six volunteers were obtained, and primary hgfs were cultured. cell viability and proliferation assay were performed to identify the ideal concentrations of csa and ang ii. cytosolic c...

Abstract Inhalation of olive pollen ( Olea europaea L.) is one the main causes allergy in Mediterranean countries and some areas North America. The response to allergens consists production inflammatory cytokines which mediated by deregulation Ca 2+ signals. In this study, biological activity material released hydration (PMR) was tested on cytosolic PE/CA-PJ15 cells (PJ-15). determined fluorome...

Cytosolic protein delivery remains elusive. The inability of most proteins to cross the cellular membrane is a huge hurdle. Here we explore unique photothermal properties gold nanorods (AuNRs) trigger cytosolic proteins. Both partners, and AuNRs, are modified with protease-resistant cell-penetrating peptide nuclear targeting induce internalization. Once internalized, spatiotemporal control rele...

Cytosolic calcium signals are evoked by a large variety of biotic and abiotic stimuli play an important role in cellular long distance signalling plants. While the function plasma membrane cytosolic Ca2+ has been intensively studied, vacuolar remains elusive. A newly developed voltage clamp technique was used combination with live-cell imaging, to study pH homeostasis bulging root hair cells Ar...

P-type H+ ATPases mediate active efflux from plant cells. They generate a proton motive force across the plasma membrane, providing free energy to drive transport of other solutes, partly by coupling influx. Wegner & Shabala (2020) recently suggested that passive influx can exceed pump-driven due ‘active buffering’, is, cytosolic scavenging and apoplastic generation metabolism (‘biochemical pH ...