methods s. cerevisiae was cultured and then disrupted by sonication. after centrifugation, the harvested supernatant was considered to be a cytoplasmic extract. the protein concentration was determined by the biuret method and the extract was diluted to concentrations of 500, 1000, and 2000 µg protein/ml. the frequencies of apoptosis and necrosis were assessed in extract-treated k562 cells by e...

The extract of pluripotent stem cells induces dedifferentiation of somatic cells with restricted plasticity. In this study, we used the extract of human embryonic stem cells (hESC) to dedifferentiate adipose tissue-derived stem cells (ADSCs) and examined the impact of this reprogramming event on dopaminergic differentiation of the cells. For this purpose, cytoplasmic extract of ESCs was prepare...

The present article relates to the rapid and efficient generation of an in vitro translational extract that is obtained from the cytoplasmic fraction of eukaryotic cells that have grown as monolayers. The procedure is totally devoid of the use of animals or animal fluids. The cytoplasmic extract that is obtained allows efficient protein synthesis of exogenously added mRNAs. The latter mRNAs can...

hit' protein profile. of different species and strains of nocardia (n.) were compared by using sixlium dodecyl sulphate - polyacryuimidc gel electrophoresis (sds-1'age) of cytoplasmic extract. in the present study n. asteroides, n. hrasilicnsis and n. otitidiscaviartim were examined. it was found that protein profiles

Vitis vinifera L.is a traditional Asian herb widely used for different health problems. In thepresent research, the ethanolic and the aqueous extracts of Vitis vinifera L. leaves collected fromshrub, grown in Tunisia, were prepared and evaluated for the antileishmanial activity againstLeishmani ainfantum promastigotes. The inhibitory concentration 50 (IC50) was determined andthe results showed ...

vitis vinifera l.is a traditional asian herb widely used for different health problems. in thepresent research, the ethanolic and the aqueous extracts of vitis vinifera l. leaves collected fromshrub, grown in tunisia, were prepared and evaluated for the antileishmanial activity againstleishmani ainfantum promastigotes. the inhibitory concentration 50 (ic50) was determined andthe results showed ...

Vitis vinifera L.is a traditional Asian herb widely used for different health problems. In thepresent research, the ethanolic and the aqueous extracts of Vitis vinifera L. leaves collected fromshrub, grown in Tunisia, were prepared and evaluated for the antileishmanial activity againstLeishmani ainfantum promastigotes. The inhibitory concentration 50 (IC50) was determined andthe results showed ...

objective: glut4 is a type of glucose transporter and plays a central role in whole-body metabolism of carbohydrates. the muscle is the major site of glut4 and cell line models, to explore glut4 behaviors under new therapeutic approach, such as herbal components, should be evaluated. here, c2c12 cell line is evaluated for glut4 translocation from intracellular compartment into the cell membrane...

Crude cytoplasmic extracts from vesicular stomatitis virus (VSV)-infected HeLa cells incorporate radioactive amino acids into hot trichloroacetic acid-precipitable material linearly for 10 to 20 min. The material synthesized in vitro corresponds in molecular weight to four of the five VSV structural proteins. However, synthesis of the viral glycoprotein (G) is significantly reduced, whereas the...