vaccination against mumps is included in world health organization (who) program of global immunization. safety and efficacy of vaccines must be confirmed by control units in charge of public health. in iran, the secondary control on potency of vaccine has not been set up yet. we decided to overcome this problem by developing who and nibsc methods in food and drug control lab. ‎nine dilutions o...

background and aims: infectious laryngotracheitis virus (iltv) infection causes a highly contagious respiratory disease in poultry leads to significant economic losses in the poultry industry. the genomic and antigenic characteristics between virulent and vaccine strains of iltv are very similar. the ability of embryonated chicken neural stem (cns) cell for differential diagnosis of iltv strain...

the ds-rna activated protein kinase (pkr) is a serine-threonine kinase with mw of 68 kda. it belongs to a family of kinases that control one of the translational initiation factors, eif2. pkr is produced at high level in response to viral infection. this protein by phosphorylating eif2 inhibits cellular protein synthesis. in this study, the effect of gamma interferon (ifn-γ), an activator, and ...

A cytopathic factor was isolated from M. hyopneumoniae VPP11 membranes. This factor had an isoelectric point of 6.2 and was capable of inducing cytopathic effect in MRC-5 human lung fibroblast culture at a concentration of 250 ng of protein per ml. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of this cytopathic factor showed a major band with a molecular weight of approximately 54,...

OBJECTIVE Clostridium difficile is a gram-positive, anaerobic, spore-forming bacillus. Usually it does not cause disease unless a patient who is colonized with toxin-producing strains has been treated with antibiotics, particularly those that change the anaerobic flora of the large intestine. METHODS We investigated in a prospective study intestinal colonization of C. difficile and its toxins...

three differentes continous cell lines (am 57. hela • vero) and primary cell culture of human embryo kidney are compared with regard to their .susceptibility for isolation of mumps v i r u s from saliva and cerebrospinal fluid. for this purfose the vero cell line appeared to be more suitable among the studied cell systems regarding the following reasons: it allows the highest pourcentage of mum...

The aetiology of the inflammatory bowel diseases. Crohn's disease, and ulcerative colitis, is still obscure. A viral aetiology has been proposed, based in part on reports that filtrates prepared from tissues of patients with inflammatory bowel disease induce cytopathic effects in tissue culture cells. Our attempts to culture viruses in many cell lines from filtrates prepared from the tissue of ...

We previously reported a rickettsial heartwater-like disease in vipers from Ghana that resembled heartwater in its gross lesions, was apparently transmitted by ticks (Aponomma and Amblyomma), and responded clinically favorably to early treatment with tetracycline. Cell culture showed consistent cytopathic effects in bovine endothelial cells, viper cells, and mouse cells, and inhibition of cytop...