Fetal chondrocytes (FCs) have recently been identified as an alternative cell source for cartilage tissue engineering applications because of their partially chondrogenically differentiated phenotype and developmental plasticity. In this study, chondrocytes derived from fetal bovine cartilage were characterized and then cultured on commercially available Cytodex-1 and Biosilon microcarriers and...

The expansion of human mesenchymal stem cells as suspension culture by means of spinner flasks and microcarriers, compared to the cultivation in tissue culture flasks, offers the advantage of reducing the requirements of large incubator capacities as well as reducing the handling effort during cultivation and harvesting. Nonporous microcarriers are preferable when the cells need to be kept in v...

The study was carried out to establish an EV71 virus culture system on Vero cells in a shake (Wave bioreactor) incorporating microcarriers for the production of hand, foot and mouth (HFM) vaccine caused by virus. results investigation 2 types (Cytodex 1 Cytodex 3), showed that gave better efficiency when culturing suspension conditions. combination with Wave bioreactor cell over 3x106cells/ml. ...

introduction: the preparation of seasonal influenza virus vaccines and especially its large-scale production requirement after the emergence or reemergence of a pandemic will need an alternative host cell system due to current suboptimal methods and the insufficiency of embryonated chicken eggs needed for producing them. in response to the vital and increasing demand for alternative means for in...

The present study outlines the synthesis of a new microcarrier for anchoragedependent animal cell cultures similar to Cytodex 1. The new microcarriers were synthesized from the cross-linking soybean starch microspheres followed by modification with 2-diethylaminoethyl (DEAE). DEAE–soybean starch microspheres were used in anchorage-dependent cell cultures using 3 g/L of the carrier and a 2.0 × 1...

The Mobius CellReady bioreactor product platform incorporates novel disposable technologies that provide optimal performance for suspension mammalian cell culture. Here we show the utility of EMD Millipore’s 3L and 50L CellReady single use bioreactors for the cultivation of adherent mammalian cells on microcarriers. Cytodex and Solohill collagen microcarriers were first tested in a mixing study...

Animal cell cultures are widely employed for the production of viral vaccines and for recombinant protein expression. The cell line Vero is a continuous, adherent cell line, which has been recommended by the World Health Organization for the production of human vaccines. For the large-scale production of vaccines, microcarriers, which are microspheres that serve as support for the cells, are be...

The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monoclonal antibody (mAb) production by CHOK1 cells. Cultures (5 ml) of microporous ...