Abstract The xylariaceous genus Dematophora has recently been resurrected and segregated from Rosellinia based on a molecular phylogeny morphological characters. This was an important taxonomic change because in the current sense contains several pathogens, while is limited to mainly saprotrophic species that have endophytic stage their life cycle may even beneficial effects host plants. During...

Submicromolar concentrations of cytochalasin inhibit the rate of assembly of highly purified dictyostelium discoideum actin, using a cytochalasin concentration range in which the final extent of assembly is minimally affected. Cytochalasin D is a more effective inhibitor than cytochalasin B, which is in keeping with the effects that have been reported on cell motility and with binding to a clas...

The action of cytochalasins, actin-disrupting agents on human Kv1.5 channel (hKv1.5) stably expressed in Ltk(-) cells was investigated using the whole cell patch-clamp technique. Cytochalasin B inhibited hKv1.5 currents rapidly and reversibly at +60 mV in a concentration-dependent manner with an IC(50) of 4.2 microM. Cytochalasin A, which has a structure very similar to cytochalasin B, inhibite...

Cytochalasin B competitively inhibits the transport of uridine and thymidine by Novikoff rat hepatoma cells growing in suspension culture with apparent K(i)'s of 2 and 6 microM, respectively, but has no effect on the intracellular phosphorylation of the nucleosides. Choline transport is not affected by cytochalasin B. Results from pulse-chase experiments indicate that cytochalasin B has no dire...

Cytochalasin B, a mold metabolite, has been shown to inhibit cytokinesis and motility (3), morphogenie development (18), endocytosis (5), and the transport of sugars (8-10, 12). I t has also been demonstrated that this drug inhibits the stimuatcd elaboration of extracellular substances from glands-the release of thyroxine from the thyroid gland after stimulation by thyroid-stimulating hormone (...

Mouse oocytes were placed in Hepes-T6 containing 7.5 μg/ml cytochalasin B and 5μg/ml Hoechst prior to enucleation, and enucleation was performed on oocytes using the manipulator. The oocyte held in place by gentle suction of a holding pipette (oocyte diameter approx.70 μm). The metaphase spindle (arrow) is aspirated into an enucleation pipette (1).

The effects of different concentrations of the actin-disrupting drug cytochalasin D on tight junction permeability and distribution of actin filaments in MDCK epithelial cells were examined. Consistent with previous studies, 2 micrograms/ml cytochalasin D caused a significant decrease in transepithelial resistance, indicative of an increase in tight junction permeability. Surprisingly, increasi...

Human blood platelets exposed to 2 UM tritiated cytochalasin D showed maximum incorporation within 5 min. Tritiated cytochalasin D was found to be bound to thrombosthenin M (platelet myosin), when subjected to gel exclusion chromatography in 0.6 M KCl. Using a Millipore filtration technique, binding at low ionic strength of tritiated cytochalasin D to purified preparations of contractile protei...