نتایج جستجو برای: cyclodextrin production
تعداد نتایج: 610639 فیلتر نتایج به سال:
Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) is an important member of α-amylase family which can degrade the starch and produce cyclodextrins (CDs) as a result of intramolecular transglycosylation (cyclization). β-Cyclodextrin production was carried out using the purified CGTase enzyme from an alkaliphile Microbacterium terrae KNR 9 with different starches in raw as well as gelatinize...
Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS–PAGE and zymographic analysis. Nine foldpurification was achieved with the specific ac...
Bacillus strains JCM 19045, JCM 19046, and JCM 19047 are alkaliphiles that produce β-cyclodextrin from starch. They are related to Bacillus xiaoxiensis and Bacillus lehensis. The genome information for these three strains will be useful for studies of the physiological role of cyclodextrin and cyclodextrin production.
Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS–PAGE and zymographic analysis. Nine foldpurification was achieved with the specific ac...
Results from the present study have shown that the ionic species of buffers, pH values and reaction temperature can affect the enzyme unit activities and product specificity of Toruzyme (Novo Nordisk A/S Bagsvaerd, Denmark) CGTase (cyclodextrin glucanotransferase). Applying a similar reaction environment (acetate buffer, pH 6.0; temperature, 60 degrees C), the CGTase was found to be capable of ...
b-cyclodextrin glucosyl transferase (b-CGTase) hydrolyses starch to produce b-cyclodextrin by transglycosylation (cyclization) activity. The conventional method for detection of b-CGTase activity is based on detecting starch hydrolysis by iodine staining. This method reveals all amylolytic enzymes, but can not discriminate them. In the present study, we introduce a new method for specific detec...
b-cyclodextrin glucosyl transferase (b-cgtase) hydrolyses starch to produce b-cyclodextrin by transglycosylation (cyclization) activity. the conventional method for detection of b-cgtase activity is based on detecting starch hydrolysis by iodine staining. this method reveals all amylolytic enzymes, but can not discriminate them. in the present study, we introduce a new method for specific detec...
background: the complexity of the fermentation processes is mainly due to the complex nature of the biological systems which follow the life in a non-linear manner. joined performance of artificial neural network (ann) and genetic algorithm (ga) in finding optimal solutions in experimentation has found to be superior compared to the statistical methods. range of applications of β-cyclodextrin (...
Cyclodextrin glycosyltransferase (CGTase) is a multifunctional enzyme that hydrolyzes the <em>α</em>-glycosidic bond between two sugar molecules and synthesizes cyclodextrins (CDs) other transglycosylation products. It ubiquitously present extracellular offers CGTase-producing organism sole right onto starch substrates over microbes. The review provides brief account of diversity am...
The process of producing cyclodextrins from starch with cyclodextrin glucanotransferase from Bacillus megaterium has been studied. The effect of starch and enzyme concentrations was evaluated using optimal composite designs and the response surface methodology. Mathematical models describing the process in two overlapping areas of variation of the independent variables were developed. The model...
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