Carbon nanotubes (CNTs) are one of the most interesting nanomaterials because of their excellent characteristics. In this study, a transparent CNTs coating for cell culture dishes was developed and its properties for cell culture were estimated. Carboxylated multiwalled carbon nanotubes (MWCNTs) were dispersed in aqueous sodium cholate solution and applied on a collagen type I-coated cell cultu...

Background: In embryological culture dishes, there is a temperature decline when they are removed outside incubators. This study aimed at investigating the effects of this temperature decline within a certain time frame, the type of culture dish with or without the use of laminar air flow and whether it is possible to achieve a sufficient thermal control with the use of a heating stage. Materia...

The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5-6 embryos/droplet) showed low blastocyst development compared with a control group (25-26 embryos/droplet). H...

In culture, myotomal muscle cells from Xenopus laevis embryos develop discrete patches of high acetylcholine receptor (AChR) density. To examine the relative adhesiveness of these sites, muscle cells having AChR patches on their lower surface (apposed to the culture dish) were identified and were then treated with dibucaine or potassium-Ringer in order to cause the cells to round up. More than ...

The effect of a magnetic field on adhesion of cultured muscle cells to the culture plate has been studied in vitro. An experimental system was manufactured to apply a magnetic field to muscle cell culture. The system consists of a couple of solenoid coils, a culture dish of 52 mm internal diameter, and an inverted phase-contrast microscope. The solenoid coil generates the alternating magnetic f...

Recently, many attractive brain-computer interface (BCI) and brainmachine interface (BMI) have been proposed. It is necessary to predict activity of a neuronal network in the brain to control an outer equipment by BCI and BMI exactly. However, it is difficult for the action potential to identify from the input-output data because a neuronal network in the brain is multilayered structure, and th...

BACKGROUND Since the identity of the embryo is of outmost importance during commercial in vitro embryo production, bovine oocytes and embryos have to be cultured strictly per donor. Due to the rather low yield of oocytes collected after ovum pick-up (OPU) per individual cow, oocyte maturation and embryo culture take place in small groups, which is often associated with inferior embryo developme...