background: melon (cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. to date, two allergen molecules, cuc m 1 and cuc m 2, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of cuc m 3. methods:the cuc m 3 cdna has been characterized by rapid amplification of cdna ends (race), which rev...

Background: Melon (Cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. To date, two allergen molecules, Cuc m 1 and Cuc m 2, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of Cuc m 3. Methods:The Cuc m 3 cDNA has been characterized by rapid amplification of cDNA ends (RACE), which ...

BACKGROUND Melon (Cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. To date, two allergen molecules, Cuc m 1 and Cuc m 2, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of Cuc m 3. METHODS The Cuc m 3 cDNA has been characterized by rapid amplification of cDNA ends (RACE), which re...

Background: Allergy is a clinical disorder affecting humans worldwide. Allergenic extracts prepared from natural source materials remain heterogeneous in composition and content, but are regularly used for diagnosis and immunotherapy. Recombinant allergens are suitable candidates to use in place of natural allergens; however, the recombinant allergens should be assessed and compared with the na...

background: allergy is a clinical disorder affecting humans worldwide. allergenic extracts prepared from natural source materials remain heterogeneous in composition and content, but are regularly used for diagnosis and immunotherapy. recombinant allergens are suitable candidates to use in place of natural allergens; however, the recombinant allergens should be assessed and compared with the na...

Cucurbitacins are a class of triterpenoids widely distributed in plant kingdom with potent anti-cancer activities both in vitro and in vivo by inducing cycle arrest, autophagy, and apoptosis. Cucurbitacin B (Cuc B), could induce S or G2/M cell cycle arrest in cancer cells while the detailed mechanisms remain to be clear. This study was designed to precisely dissect the signaling pathway(s) resp...

Cucurbitacin B (Cuc B), a natural product, induced both protective autophagy and DNA damage mediated by ROS while the detailed mechanisms remain unclear. This study explored the mechanism of Cuc B-induced DNA damage and autophagy. Cuc B decreased cell viability in concentration- and time-dependent manners. Cuc B caused long comet tails and increased expression of γ-H2AX, phosphorylation of ATM/...

BACKGROUND Chronic ulcerative colitis (CUC) is associated with increased risk of developing colon cancer through a dysplasia (intraepithelial neoplasia)-carcinoma sequence. AIMS To investigate the expression of apoptosis and inflammatory related proteins in CUC. METHODS The expression of proteins involved in apoptosis and inflammation (inducible nitric oxide synthase (iNOS), cyclooxygenase ...

background: several studies reported the clinical features of ige-mediated hypersensitivity after ingestion of melon. melon allergy is a common ige-mediated fruit allergy in iran. this prompted us to investigate immunochemical and molecular properties of the major allergen in melon fruit, to compare the ige-binding capacity of the natural protein with the recombinant allergen, and to determine ...