Background: The prevalence of ESBLs-producing Escherichia coli has recently increased worldwide due to the expression ESBL genes which had led high rate multidrug resistance antibiotics. Aim: To determine antimicrobial susceptibility patterns E. isolates and evaluating carriage these at phenotypic genotypic levels. Methods: One hundred two clinical were recovered from UTI suspects analyzed for ...

Technical Appendix Table. β-lactamase profiles of multidrug-resistant Gram-negative bacilli isolated in 2010 and 2011, France* Region of origin No. culture-positive patients/total no. patients Species No. isolates β-lactamase ESBL Carbapenemase pCASE Africa 33/87 E. coli 18 CTX-M-15 2 CTX-M-1 2 SHV-12 1 CTX-M-3 OXA-48 1 CTX-M-14 1 CTX-M-15 TEM-169 1 CTX-M-15 CMY-2 1 CTX-M-24 OXA-48 1 ND K. pneu...

The A2 chain of cholera toxin (CTX) contains a COOH-terminal Lys-Asp-Glu-Leu (KDEL) sequence. We have, therefore, analyzed by immunofluorescence and by subcellular fractionation in Vero cells whether CTX can used to demonstrate a retrograde transport of KDEL proteins from the Golgi to the ER. Immunofluorescence studies reveal that after a pulse treatment with CTX, the CTX-A and B subunits (CTX-...

Abstract Infection of the urinary tract ranks as one most common infections affecting people worldwide and its treatment is made complicated by rising incidence antibiotic resistance. This study aimed to detect extended spectrum beta-lactamase (ESBL) genes resistance profile uropathogenic Escherichia coli ( E. ) recovered from patients attending a University Teaching hospital in Nigeria. Uropat...

Toxigenic Vibrio cholerae strains arise upon infection and integration of the lysogenic cholera toxin phage, the CTX phage, into bacterial chromosomes. The V. cholerae serogroup O1 strains identified to date can be broadly categorized into three main groups: the classical biotype strains, which harbor CTX-cla; the prototype El Tor strains (Wave 1 strains), which harbor CTX-1; and the atypical E...

Infections of CTX-M extended-spectrum β-lactamase-producing Enterobacterales are a severe threat in clinical settings. genes on plasmids have been transferred to many species, and these species spread, leading the global problem antimicrobial resistance. Here, we developed lateral flow immunoassay (LFIA) based an anti-CTX-M rabbit monoclonal antibody. This antibody detected variants from CTX-M-...

Background Uropathogenic Escherichia coli (UPEC) is the most important pathogen involved in urinary tract infections (UTIs). Beta-lactam antibiotics have been widely used to treat UPEC infections; however, extended-spectrum beta-lactamases (ESBLs) create significant therapeutic problems by inactivating almost all beta-lactams except cephamycins and carbapenems [1,2]. The CTX-M-type beta-lactama...