The purpose of the present study was to perform bioinformatic analysis crystallin diversity in aquatic molluscs based on sequences NCBI Protein database. objectives were as follows: (1) some physical and chemical properties mollusc crystallins, (2) comparison crystallins with zebrafish cubomedusa Tripedalia cystophora (3) determination most probable candidates for role gastropod eye crystallins...

Crystallins are the predominant proteins of eye lens which prevent heat and oxidative-induced stress-induced aggregation other proteins. They may be classified into two superfamilies, α- βγ- crystallins. The crystallins long-lived structural refract light onto retina. microbial can not only bind to calcium ions, but even able coordinate ions such as Mg2+, Sr2+, Co2+, Mn2+, Ni2+, Zn2+ etc. Such ...

Purtx~se: We like to demonstrate glycation of S,-, R,and y-crystallins in the microsectioned bovine lens, by isoelectric focusing using three staining methods. Methods: Pour bovine lenses of 1.185+0.131 years were frozen-sectioned into equa'cor and 11 layers. Watersoluble crvstallins were seuarated bv isoelectric focusing an8 stained: 1. with ?oomassie brilliant Blue (CBB); 2. with the lectin (...

PURPOSE Previous theoretical and experimental studies have predicted that the loss of weak protein interactions between alpha- and gamma-crystallins could result in a decrease in the transparent properties of the aging lens. METHODS alpha-Crystallins were prepared from the nucleus of old bovine lens, and gamma-crystallins were prepared from whole fetal bovine lens or from the nucleus of old b...

Purtx~se: We like to demonstrate glycation of S,-, R,and y-crystallins in the microsectioned bovine lens, by isoelectric focusing using three staining methods. Methods: Pour bovine lenses of 1.185+0.131 years were frozen-sectioned into equa'cor and 11 layers. Watersoluble crvstallins were seuarated bv isoelectric focusing an8 stained: 1. with ?oomassie brilliant Blue (CBB); 2. with the lectin (...

Purtx~se: We like to demonstrate glycation of S,-, R,and y-crystallins in the microsectioned bovine lens, by isoelectric focusing using three staining methods. Methods: Pour bovine lenses of 1.185+0.131 years were frozen-sectioned into equa'cor and 11 layers. Watersoluble crvstallins were seuarated bv isoelectric focusing an8 stained: 1. with ?oomassie brilliant Blue (CBB); 2. with the lectin (...

Purtx~se: We like to demonstrate glycation of S,-, R,and y-crystallins in the microsectioned bovine lens, by isoelectric focusing using three staining methods. Methods: Pour bovine lenses of 1.185+0.131 years were frozen-sectioned into equa'cor and 11 layers. Watersoluble crvstallins were seuarated bv isoelectric focusing an8 stained: 1. with ?oomassie brilliant Blue (CBB); 2. with the lectin (...

PURPOSE How corneal transparency is formed/maintained remains largely unclear. A group of enzymes which are referred to as enzymatic crystallins were proposed to contribute to corneal transparency in various animals. This study investigated whether the three classical lens crystallins, namely α-, β-, and γ-crystallins, exist in mouse and human corneas. METHODS Mice, human tissues, and culture...

PURPOSE The purpose of the study was to compare and analyze the composition of crystallin species that exist in the water insoluble-urea soluble (WI-US) and water insoluble-urea insoluble (WI-UI) protein fractions of a human cataractous lens and an age-matched normal lens. METHODS The water soluble (WS) and water insoluble (WI) protein fractions from a 68-year-old normal lens and a 61-year-ol...

PURPOSE Changes in lens protein expression during zebrafish development results in a smooth gradient of refractive index necessary for excellent optical function. Age-related changes in crystallin expression have been well documented in mammals but are poorly understood in the zebrafish. METHODS In the zebrafish lens, a systematic analysis of protein content with age was performed using size ...