background: human sperm cryopreservation has proven to be very valuable. cryopreservation has effects on function, morphology and percentage of fertilization capacity of human sperm. also, it has been revealed that cryopreservation has a role in sperm dna fragmentation and infertility. in this study, viability, motility, dna fragmentation and for the first time, intracellular nitric oxide asses...

Human pluripotent stem cell (hPSC)-derived cell therapy requires production of therapeutic cells in large quantity, which starts from thawing the cryopreserved cells from a working cell bank or a master cell bank. An optimal cryopreservation and thaw process determines the efficiency of hPSC expansion and plays a significant role in the subsequent lineage-specific differentiation. However, cryo...

protectant for spermatozoa41) led to research on cryopreservation of mammalian embryos. In the past quarter of a century since the first successful cryopreservation of mouse embryos58,60), basic and applied research has resulted in the cryopreservation of embryos of many mammalian species. Improvements have been made in nearly all steps of the cryopreservation process, resulting in considerable...

Despite the significant progress, the post-thaw viability and fertility of the cryopreserved sperm are still reduced, as a consequence of accumulated cellular injuries that arise throughout the cryopreservation process. Many laboratory tests have already been carried out to verify these detrimental effects and their origin. Their is needed to well understand the whole process of cryopreservatio...

The effects of cryopreservation on mesenchymal stem cell (MSC) phenotype are not well documented; however this process is of increasing importance for regenerative therapies. This study examined the effect of cryopreservation (10% dimethyl-sulfoxide) on the morphology, viability, gene-expression and relative proportion of MSC surface-markers on cells derived from rat adipose, bone marrow and de...

Vitrification and slow-freezing methods have been used for the cryopreservation of human pluripotent stem cells (hPSCs). Vitrification requires considerable skill and post-thaw recovery is low. Furthermore, it is not suitable for cryopreservation of large numbers of hPSCs. While slow-freezing methods for hPSCs are easy to perform, they are usually preceded by a complicated cell dissociation pro...

In modern livestock breeding, cryopreserved semen is routinely used for artificial insemination. Sperm cryopreservation secures future reproduction, and insemination doses can be easily shipped. Processing of semen for cryopreservation can be done with minimal efforts and relatively low costs. In this chapter we describe the entire cryopreservation process for stallion and bull sperm including ...

introduction: infertility is a common phenomenon in modern societies. today, use of assisted reproductive technologies (arts) for treatment of infertility is common, and cryopreservation is one of these technologies. cryopreservation has impact on the function and percentage of fertility of human sperm. in this study, motility, viability, nitric oxide, and dna apoptosis were assessed before and...

Cryopreservation is required to preserve the native properties of tissue for prolonged periods time. In this study, we evaluate impact that 4 different cryopreservation protocols have on porcine urethral tissue, identify a protocol best preserves tissue. The include storage in cryoprotective agents at ?20 °C and ?80 with slow, gradual, fast reduction temperature. To effects cryopreservation, me...