two experiments were conducted to evaluate the crude protein fractionation, in vitro gas production and the biological effects of tannins in alfalfa silage treated with different levels of pistachio by-products (pb) extract. pb was soaked in water with a ratio of 1:5 (w/v) for 12 h. after filtering and concentrating, the crude extract was added to fresh alfalfa to a final concentration of 1%, 2...

Denaturing Isoelectric Focusing Free-Flow Electrophoresis (IEF-FFE) Fractionation of Crude Protein Mixtures Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn This protocol was adapted from "Denaturing IEF-FFE Fractionation of Crude Protein Mixtures," contributed by Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn, Chapter 19, in Purifying Proteins for Proteomics (ed. Simpson). ...

Native Isoelectric Focusing Free-flow Electrophoresis (IEF-FFE) Fractionation of Crude Protein Mixtures Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn This protocol was adapted from "Native IEF-FFE Fractionation of Crude Protein Mixtures," contributed by Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn, Chapter 19, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spr...

Two experiments were conducted to evaluate the crude protein fractionation, in vitro gas production and the biological effects of tannins in alfalfa silage treated with different levels of pistachio by-products (PB) extract. PB was soaked in water with a ratio of 1:5 (w/v) for 12 h. After filtering and concentrating, the crude extract was added to fresh alfalfa to a final concentration of 1%, 2...

Platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of Cerastes persicus fieldi and Echis carinatus were investigated. The partial fractionation was performed on a Sephadex G-100 column. Two fractions separated from Cerastes persicus fieldi showed anti platelet aggregation activity on ADP (200 µM)-induced platelet aggregation (ca 80% inhibi...

Platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of Cerastes persicus fieldi and Echis carinatus were investigated. The partial fractionation was performed on a Sephadex G-100 column. Two fractions separated from Cerastes persicus fieldi showed anti platelet aggregation activity on ADP (200 µM)-induced platelet aggregation (ca 80% inhibi...

The changes in crude protein, free amino acids, amino acid composition, protein solubility, protein fractionation and protein digestibility after germination of sorghum were investigated. Sorghum varieties (Dorado, Shandaweel-6, Giza-15) were soaked for 20 h followed by germination for 72 h; the results revealed that crude protein and free amino acids in raw sorghum varieties ranged from 10.62 ...

platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of cerastes persicus fieldi and echis carinatus were investigated. the partial fractionation was performed on a sephadex g-100 column. two fractions separated from cerastes persicus fieldi showed anti platelet aggregation activity on adp (200 µm)-induced platelet aggregation (ca 80% inhibi...

background: there are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. there are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...