Sarcosine oxidase (EC 1.5.3.1; sarcosine:oxygen oxidoreductase [demethylating]) is a flavoprotein that catalyses the oxidative demethylation of sarcosine to yield glycine, formaldehydethe, and hydrogen peroxide. This enzyme is involved in the bacterial metabolism of creatinine with the related enzymes, creatininase and creatinase. It is industrially important and is used with creatininase and c...

Creatinine amidohydrolase(EC 3.5.2.10) catalyzes the reversible conversion of creatinine to creatine. Creatininase in combination with other enzymes is used for detection of creatinine in serum and urine which is of significant value for detection of renal, muscular and thyroid functions. The aim of this study was to produce recombinant creatininase enzyme in E.coli expression system to use it ...

“Creatininase” activity was induced in the colon flora of rats by feeding creatinine. Sarcosine and methylamine were tentatively identified as the major products of the enzymic action. A third (unidentified) compound was observed. These compounds and methylguanidine were labeled in the urine of rats given ‘CH3-creatinine orally. The resuits suggest that creatinine should not be considered biolo...

Novel biosensors, based on immobilized creatininase, creatinase and urease are developed, using ISFETs with weak inversion at pH 6-8 and 37.0C. The ISFETs with circuitry, demonstrate a linear relationship of urea and creatinine at the range of 0-200 mM and 0-20 mM, respectively. Preliminary results show that biosensors operating in weak inversion mode can eliminate many of the disadvantages of ...

The lack of any breakdown of urea under the experimental conditions led to the conclusion that urease was not present in streptococci and that urea could not be an intermediate in the reaction. Thus it seems likely that the formation of ornithine from arginine by these bacteria occurred by a pathway different from that possessed by arginase of mammalian liver. Niven et al. (1942) tested the abi...

A two-step method for assaying creatinine in serum and urine samples, suitable with automated analyzers, is reported. Reagent 1, for the first step, contains a blanking system [creatine amidinohydrolase (CRTase), urease, glutamate dehydrogenase, NADPH, and 2-oxoglutarate] and a NADPH-regenerating system [Mg(2+)-dependent isocitrate dehydrogenase (ICD), MgCl2, and excess isocitrate]. Reagent 2, ...

An enzyme electrode system for the determination of creatinine and creatine was developed by utilizing three enzymes: creatinine amidohydrolase (CA), creatine amidinohydrolase (Cl), and sarcosine oxidase (SO). These enzymes were co-immobilized onto the porous side of a cellulose acetate membrane with asymmetric structure, which has selective permeability to hydrogen peroxide. Two kinds of multi...