diagnosis of corneal diseases is possible by measuring and evaluation of corneal thickness in different layers. thus, the need for precise segmentation of corneal layer boundaries is inevitable. obviously, manual segmentation is time‑consuming and imprecise. in this paper, the gaussian mixture model (gmm) is used for automatic segmentation of three clinically important corneal boundaries on opt...

The authors examined immunofluorescently the specific cytokeratin staining patterns of corneal, limbal, and conjunctival epithelium with PKK-1, 8.12, 4.62, and 8.60 monoclonal anticytokeratin antibodies. Observations were made on unfixed frozen postmortem human tissue. The PKK-1 antibody stain was observed in all layers of corneal epithelium but only in suprabasal layers of limbal and conjuncti...

Hypoxia induced corneal swelling was observed and evaluated in healthy human volunteers by use of high speed, ultrahigh resolution optical coherence tomography (UHROCT). Two dimensional corneal images were acquired at a speed of 47,000 A-scans/s with 3μm x 10μm (axial x lateral) resolution in corneal tissue. The UHROCT tomograms showed clear visualization of all corneal layers, including the Bo...

due to the high economic value of ostrich and the farming industry to further promote increased productivity, knowledge in different fields of science, including detailed knowledge of tissue structures vital organs from this animal looks. ostrich’s eye is the cornea closest to the human eye because of the same anatomical features of the human cornea, corneal transplantation can be used ostrich....

PURPOSE To determine the UV absorbance of the corneal layers (epithelium, Bowman layer, stroma) in the 240- to 400-nm range. METHODS Consecutive slices (100 microm) of human cadaveric corneas were cut, and the UV absorbance of each sample was determined in a scanning spectrophotometer. In some cases the epithelium was scraped off and its absorbance measured separately. RESULTS The investiga...

PURPOSE To describe bilateral corneal alterations through confocal microscopy in a patient affected by Cowden syndrome (CS). METHODS Evaluation of Schirmer's, fluorescein, and lissamine green dye tests. Confocal microscopy was performed in both eyes to investigate corneal abnormalities. RESULTS Slit lamp observation showed the focal involvement of anterior stromal and epithelial layers. Sch...

Maeder and Datiis (1947) described a 40-year-old woman with small white curved filaments occupying a disc in the deepest layers of the corneal stroma. The peripheral 2 mm. of cornea remained unaffected. The corneae of this patient showed keratoconus, clinically manifest in the left eye with a forme fruste in the right. D'Ermo (1950) annotated a similar case in a 19-year-old girl who was also af...

PURPOSE We describe a case of full-thickness corneal restoration after an acute corneal burn with an acid agent. METHODS A 32-year-old male reported painful discomfort, redness, photophobia, and a decrease in visual acuity in the left eye after a unilateral burn with an acid agent. Slit-lamp examination revealed massive corneal melting involving necrotic sequestrum of the entire corneal surfa...

PURPOSE The present study was undertaken to create a conditional knockout of AP-2alpha in the corneal epithelium. METHODS A line of mice expressing Cre-recombinase specifically in the early lens placode was crossed with mice in which the AP-2alpha allele is flanked by two loxP sites. The resultant Le-AP-2alpha mutants exhibited a targeted deletion of AP-2alpha in lens placode derivatives, inc...

Conventional corneal transplantation, in the form of penetrating keratoplasty (PK), involves full-thickness replacement of the cornea, and is a highly successful procedure. However, the cornea is anatomically a multi-layered structure. Pathology may only affect individual layers of the cornea, hence selective lamellar surgical replacement of only the diseased corneal layers whilst retaining una...