infection with hepatitis c virus (hcv) is a worldwide problem. among hcv proteins, core antigen (ag), besides its importance for diagnostic application is a prime candidate for component of a vaccine. herein, we report results of studies on production of the hydrophilic domain of core ag (2-122) in native conformation by an arabinose induction system in e.coli and the primary characterization o...

conclusions the canola oilseeds could provide a useful expression system to produce hcv core protein as a vaccine candidate. results western blot analysis using anti-his antibody confirmed the presence of a 15 kda protein in the seeds of t1 transgenic lines. the amount of antigenic protein accumulated in the seeds of these transgenic lines was up to 0.05% of the total soluble protein (tsp). mat...

the polymerase chain reaction (pcr) was used to amplify a 578 bp fragment of the poxvirus 4b core protein. avipoxvirus (apv) specific dna was detected in all 10 different isolates (each of which had been isolated from an epidemic) isolated from chicken; canary and mynah were collected from tehran province. sequencing was performed for 2 isolates as representative and the nucleotide sequence sho...

conclusions there is an as yet unexplained association between hcv and host parameters with unknown mechanisms in patients with chronic hcv infection. the assessments of core aa 70 substitution and polymorphisms near the ifnl3 gene could offer promising steps to improve the management of patients with hcv. background molecular studies have demonstrated that the hepatitis c virus (hcv) genotype ...

background: hepatitis c virus (hcv) is a main public health problem causing chronic liver infection and subsequently liver cirrhosis and lethal hepatocellular carcinoma (hcc). vaccination based on hcv capsid proteins has attracted a special interest for prevention of viral infections. the core protein is a basic and evolutionary most conserved protein, which regulates the cellular processes rel...

Introduction: Hepatitis C virus (HCV) infection is a major public health problem which influences about 170 million people worldwide. Different types of vaccines have been designed using HCV structural proteins to control the viral infection. The core nucleocapsid protein is one of the most conserved proteins and a desirable target for HCV vaccines, especially the protein-based vaccines. In cur...

Background: Hepatitis C virus (HCV) is a main public health problem causing chronic liver infection and subsequently liver cirrhosis and lethal hepatocellular carcinoma (HCC). Vaccination based on HCV capsid proteins has attracted a special interest for prevention of viral infections. The core protein is a basic and evolutionary most conserved protein, which regulates the cellular processes rel...

background the hepatitis c virus (hcv) infection has been identified as a leading cause of progressive liver diseases worldwide. despite new treatment strategies, pegylated interferon alfa-2a (peg-ifnα-2a), in combination with ribavirin (rbv), still represents the gold standard of therapy for hepatitis c in developing countries. results in this study, a significant association was observed betw...

introduction: hepatitis c virus (hcv) infection is a major public health problem which influences about 170 million people worldwide. different types of vaccines have been designed using hcv structural proteins to control the viral infection. the core nucleocapsid protein is one of the most conserved proteins and a desirable target for hcv vaccines, especially the protein-based vaccines. in cur...