cord blood is a rich source of hematopoietic stem cells and could be potentially used for transplantation instead of conventional sources of stem cells (bone marrow or peripheral blood). cord blood cells are successfully used in pediatric and adult patients, but their major limitation is the low number of hematopoietic stem cells for patients of large body size. there are several possible solut...

umbilical cord blood (ucb) has been used for transplantation in the treatment of hematologic disorders as a source of hematopoietic stem cells (hscs). because of insufficient number of cord blood cd34+ cells, the expansion of these cells seems to be important for clinical application. mesenchymal stromal cells (mscs), playing an important role in hscs maintenance, were used as feeder layer. apo...

Umbilical cord blood (UCB) has been used for transplantation in the treatment of hematologic disorders as a source of hematopoietic stem cells (HSCs). Because of insufficient number of cord blood CD34+ cells, the expansion of these cells seems to be important for clinical application. Mesenchymal stromal cells (MSCs), playing an important role in HSCs maintenance, were used as feeder layer. Apo...

introduction: umbilical cord blood (cb) has been identified as a rich source for hematopoietic stem cells (hscs), and has provided an alternative to bone marrow transplantation. the use of ex vivo expanded cells has been suggested as a possible means to accelerate the speed of engraftment in cord blood (cb) transplantation. the main aim of our study is to find the best culture media and conditi...

Background: The mammalian target of rapamycin (mTOR) is important in hematopoiesis. Despite the central role of mTOR in regulating the differentiation of immune cells, the effect of mTOR function on cord blood mononuclear cells is yet to be defined. Objectives: To evaluate the effect of mTOR inhibition, using rapamycin on the proliferation and apoptosis of cord blood mononuclear cells, as well ...

introduction: human hematopoietic stem cells (hhscs) have been used for transplantation in hematologic failures. because the number of hhscs per cord blood unit is limited, the expansion of these cells is important for clinical application. it has been reported that cytokines and feeder layer provide a perspective to in vitro expansion of hhscs. in this regard, cord blood cd34+ cells expanded a...

Introduction: Human hematopoietic stem cells (hHSCs) have been used for transplantation in hematologic failures. Because the number of hHSCs per cord blood unit is limited, the expansion of these cells is important for clinical application. It has been reported that cytokines and feeder layer provide a perspective to in vitro expansion of hHSCs. In this regard, cord blood CD34+ cells ex...

background the aim of this study was the ex vivo expansion of umbilical cord blood hematopoietic stem cells on biocompatible nanofiber scaffolds. materials and methods cd133+ hematopoietic stem cells were separated from umbilical cord blood using midimacs (positive selection) system by means of monocolonal antibody cd133 (microbeads) subsequently, flowcytometry method was done to assess the pur...