Detection of antigens in faecal supernatants is a more reliable and suitable method, in cases where coprological examination fails to reveal any parasitic ova. Hundred cattle known to be infected with Gastrothylax crumenifer, the most prevalent amphistome species were selected at random for the study. An indirect ELISA was performed to detect coproantigens in faecal supernatants of these infect...

BACKGROUND The study was targeted to report the appearance of coproantigens in feces and circulating antibodies in the serum of Fasciola gigantica experimentally infected rabbits. METHODS Copro Hyper Immune Serum (HIS) and Excretory-Secretory Hyper Immune Serum (ES HIS) antigens were used in a sandwich ELISA for the detection of F. gigantica antigens in feces of 12 rabbits experimentally infe...

Two monoclonal antibodies to Fasciola gigantica excretory/secretory (ES) antigens were used in a sandwich ELISA for the detection of Fasciola antigens in faeces of 9 sheep experimentally infected with 300 metacercariae of F. gigantica. The detection of coproantigens was found in four of the seven sheep within 5 weeks of infection, and within 7 weeks of infection coproantigens were detected in a...

In the present study the dynamics of antigenemia and coproantigens were studied in patients with Fasciola hepatica infection during an outbreak occurring in La Palma, Pinar del Río, in the West Province of Cuba. Stool and serum samples were collected from 67 patients and 40 healthy subjects. Stool samples were studied by a simple gravity sedimentation technique and an ES78 sandwich enzyme-linke...

A sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Echinococcus multilocularis coproantigens (EM-ELISA) was developed with polyclonal rabbit (solid phase) and chicken egg (catching) antibodies that were directed against E. multilocularis coproantigens and somatic worm antigens, respectively. In experimentally infected dogs and cats, coproantigens were first detectable 6-1...

:  In the present study, 90 coprolites from La Cueva de los Muertos Chiquitos (CMC) were subjected to enzyme-linked immunosorbent assay (ELISA) tests for 3 diarrhea-inducing protozoan parasites, Entamoeba histolytica , Giardia duodenalis , and Cryptosporidium parvum , to determine whether these parasites were present among the people who utilized this cave 1,200-1,400 yr ago. These people, the ...

The objective of present study was to determine the prevalence of natural caprine fasciolosis in the Mixteca region of Mexico using coproantigen and serum IgG1 ELISA tests for comparative purposes. A total of 1070 serum and faecal samples were analyzed for IgG1 antibodies and coproantigens, using ELISA with E/S products as antigen and a monoclonal antibody-based sandwich ELISA. Prevalence of 73...

background: the study was targeted to report the appearance of coproantigens in feces and circulating antibodies in the serum of fasciola gigantica  experimentally infected rabbits. methods: copro hyper immune serum (his) and excretory-secretory hyper immune serum (es his) antigens were used in a sandwich elisa for the detection of f. gigantica antigens in feces of 12 rabbits experimentally inf...