copper amine oxidases (caos) catalyse the oxidative de-amination of biogenic amines which are ubiquitous compounds essential for cell growth and proliferation. the enzymes are homodimers containing both topaquinone and a cu(ii) ions as cofactors at the active site of each subunit. after extraction and purification of chickpea (cicer arietinum) amine oxidase by chromatoghraphy, km and vmax of th...

copper amine oxidases are important enzymes, which contribute to the regulation of mono- and polyamine levels. each monomer contains one cu(ii) ion and 2,4,5-trihydroxyphenylalanine (tpq) as cofactors. they catalyze the oxidative deamination of primary amines to aldehydes with a ping-pong mechanism consisting of a transamination. the mechanism is followed by the transfer of two electrons to mol...

Copper amine oxidases (CAOs) catalyse the oxidative de-amination of biogenic amines which are ubiquitous compounds essential for cell growth and proliferation. The enzymes are homodimers containing both topaquinone and a Cu(II) ions as cofactors at the active site of each subunit. After extraction and purification of chickpea (cicer arietinum) amine oxidase by chromatoghraphy, Km and Vmax of th...

living organisms. This class of enzymes is divided into two subclasses: FAD-containing amine oxidases (FAD-AOs) and Cu-containing amine oxidases (Cu-AOs). Two types of mitochondrial monoamine oxidases, monoamine oxidases (MAO)A and MAO-B, and cytosolic polyamine oxidase are included in FAD-AOs. The Cu-AOs consist of retina amine oxidase, diamine oxidase (DAO), and semicarbazide-sensitive amine ...

Nanotechnology for cancer gene therapy is an emerging field. Nucleic acids, polyamine analogues and cytotoxic products of polyamine oxidation, generated in situ by an enzyme-catalyzed reaction, can be developed for nanotechnology-based cancer therapeutics with reduced systemic toxicity and improved therapeutic efficacy. Nucleic acid-based gene therapy approaches depend on the compaction of DNA/...

Spermine was toxic to BHK-21/C13 cells in the absence of any extracellular metabolism of the amine. Inhibition of copper-containing amine oxidases with aminoguanidine partially prevented the response, whereas inhibition of polyamine oxidase with MDL-72,527 exacerbated the effect. Oxidation by an intracellular copper-containing amine oxidase may be involved in the toxicity of spermine, whereas t...