نتایج جستجو برای: connexin43

تعداد نتایج: 1453  

Journal: :The Journal of Cell Biology 1991
L S Musil D A Goodenough

We previously demonstrated that the gap junction protein connexin43 is translated as a 42-kD protein (connexin43-NP) that is efficiently phosphorylated to a 46,000-Mr species (connexin43-P2) in gap junctional communication-competent, but not in communication-deficient, cells. In this study, we used a combination of metabolic radiolabeling and immunoprecipitation to investigate the assembly of c...

Journal: :Journal of cell science 2006
Qingyi Zheng-Fischhöfer Alexander Ghanem Jung-Sun Kim Mark Kibschull Gaby Schwarz Jörg O Schwab James Nagy Elke Winterhager Klaus Tiemann Klaus Willecke

In the gastrulating mouse embryo, the gap junction protein connexin43 is expressed exclusively in cells derived from the inner cell mass, whereas connexin31 is expressed in cells of the trophoblast lineage. Since connexin43 and connexin31 do not form heterotypic gap junction channels in exogenous expression systems, such as HeLa cells and Xenopus oocytes, previous studies have suggested that th...

Journal: :The Journal of Cell Biology 1990
L S Musil B A Cunningham G M Edelman D A Goodenough

Connexin43 is a member of the highly homologous connexin family of gap junction proteins. We have studied how connexin monomers are assembled into functional gap junction plaques by examining the biosynthesis of connexin43 in cell types that differ greatly in their ability to form functional gap junctions. Using a combination of metabolic radiolabeling and immunoprecipitation, we have shown tha...

2017
Odunayo O Mugisho Colin R Green Jie Zhang Nicolette Binz Monica L Acosta Elizabeth Rakoczy Ilva D Rupenthal

Diabetic retinopathy (DR) develops due to hyperglycemia and inflammation-induced vascular disruptions in the retina with connexin43 expression patterns in the disease still debated. Here, the effects of hyperglycemia and inflammation on connexin43 expression in vitro in a mouse model of DR and in human donor tissues were evaluated. Primary human retinal microvascular endothelial cells (hRMECs) ...

Journal: :Journal of cell science 1998
P D Lampe W E Kurata B J Warn-Cramer A F Lau

The gap junction protein connexin43 is a phosphoprotein that typically migrates as three bands (nonphosphorylated, P1 and P2) during polyacrylamide gel electrophoresis. The electrophoretic mobility of connexin43 from mitotic cells was distinctly reduced to a form (P3) that migrated slower than P2 from Rat1 cells prepared by shakeoff of nocodazole-treated and untreated cultures. Mitotic FT210 ce...

Journal: :Arteriosclerosis, thrombosis, and vascular biology 2001
Y S Ko S R Coppen E Dupont S Rothery N J Severs

The gap-junctional protein, connexin43, is differentially expressed in vascular smooth muscle cells (SMCs) according to phenotype. Previous studies suggest that desmin-negative SMCs are characterized by high levels of connexin43, whereas desmin-positive SMCs (of a more contractile phenotype) typically have low connexin43 levels. In this study, we examine systematically the inverse relationship ...

Journal: :Journal of cell science 2012
Tone Aase Fykerud Ane Kjenseth Kay Oliver Schink Solveig Sirnes Jarle Bruun Yasufumi Omori Andreas Brech Edgar Rivedal Edward Leithe

Gap junctions consist of arrays of intercellular channels that enable adjacent cells to communicate both electrically and metabolically. Gap junction channels are made of a family of integral membrane proteins called connexins, of which the best-studied member is connexin43. Gap junctions are dynamic plasma membrane domains, and connexin43 has a high turnover rate in most tissue types. However,...

Journal: :Brain : a journal of neurology 2012
Helen V Danesh-Meyer Nathan M Kerr Jie Zhang Elizabeth K Eady Simon J O'Carroll Louise F B Nicholson Cameron S Johnson Colin R Green

Connexin43 gap junction protein is expressed in astrocytes and the vascular endothelium in the central nervous system. It is upregulated following central nervous system injury and is recognized as playing an important role in modulating the extent of damage. Studies that have transiently blocked connexin43 in spinal cord injury and central nervous system epileptic models have reported neuronal...

Journal: :Development 2002
W E I Li K Waldo K L Linask T Chen A Wessels M S Parmacek M L Kirby C W Lo

Connexin43 knockout mice die neonatally from conotruncal heart malformation and outflow obstruction. Previous studies have indicated the involvement of neural crest perturbations in these cardiac anomalies. We provide evidence for the involvement of another extracardiac cell population, the proepicardial cells. These cells give rise to the vascular smooth muscle cells of the coronary arteries a...

Journal: :Journal of cell science 2003
Marc A Thomas Nathalie Zosso Isabelle Scerri Nicolas Demaurex Marc Chanson Olivier Staub

The gap junction protein connexin43 is known to have a rapid turnover, involving degradation by both the proteasomal and lysosomal systems, but the structural features of connexin43 that govern these actions are not known. The connexin43 C-terminal sequence contains a proline-rich region corresponding to the consensus of a protein-protein interaction PY-motif (xPPxY), and an overlapping putativ...

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