A label-free-based fiber optic biosensor based on etched tilted Bragg grating (TFBG) is proposed and practically demonstrated. Conventional phase mask technic has been utilized to inscribe with a tilt angle of 10°, while the etching accomplished hydrofluoric acid. composite polyethylenimine (PEI)/poly(acrylic acid) (PAA) thermally deposited TFBG, followed by immobilization probe DNA (pDNA) this...

A label-free colorimetric method based on exonuclease I (Exo I)-assisted signal amplification with protamine as a medium was developed for analysis of kanamycin. In this study, double-stranded DNA (dsDNA) probe tailored by manipulating an aptamer and its complementary (cDNA) ensuring detection target high selectivity excellent sensitivity. Herein, could not only combine negatively charged gold ...

Most cDNA library screening procedures do not distinguish between full-length and incomplete clones and therefore may yield incomplete cDNA fragments. Thus, there is a widespread need for a method allowing the efficient selection of full-length clones. I present a rapid, PCR-based method that allows the simultaneous screening of > 10(6) cDNAs. The longest cDNA is identified in the first step so...

One challenge in point-of-care (POC) diagnostics is the lack of room-temperature methods for RNA detection based on enzymatic amplification and visualization steps. Here we perform reverse transcription lesion-induced DNA (RT-LIDA), an isothermal method that only requires T4 ligase. RT-LIDA involves RNA-templated ligation primers to form complementary (cDNA) followed by toehold-mediated strand ...

Suppression subtractive hybridization (SSH) is a widely used method for separating DNA molecules that distinguish two closely related DNA samples. Two of the main SSH applications are cDNA subtraction and genomic DNA subtraction. In fact, SSH is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. The SSH method is based on a suppression PCR effe...

The yeast three-hybrid system can be used to identify a protein partner of a known RNA sequence by screening a cDNA library fused to a transcription activation domain, with a hybrid RNA as 'bait.' Most commonly, such screens are performed to identify proteins that interact with a given RNA in vivo.

As previously shown, purified 14S RNA of mouse myeloma MOPC-41 forms a single peak on sucrose gradients and gel electrophoresis and codes for a single polypeptide chain, the immunoglobulin light chain produced by the same myeloma in vivo. This 14S mRNA was used for the enzymatic synthesis of DNA (cDNA) which is complementary to the RNA template. A DNA fraction was isolated which has an average ...