to evaluate pathogenicity of 3 chicken anemia virus isolates-cv1, cv2 and cv3 respectively - the current experiment carried out. after tittering of the viruses, 30 one day old spf chicks were grouped and intramuscularly inoculated with each isolate in a separate group. two other groups as positive and control groups were inoculate with a live vaccine virus and normal saline respectively. during...

BACKGROUND Brucellosis is the most common bacterial zoonosis, and serological tests are routinely used in brucellosis control and eradication programs. In order to improve the accuracy of serological diagnostic method used in bovine brucellosis detection, this study developed an improved competitive ELISA with higher specificity and good sensitivity. RESULTS This study prepared 12 monoclonal ...

COMPARISON OF ENZYME IMMUNOASSAYS FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS. The indirect enzyme immunoassay for measurement of bovine antibody to Brucella abortus was tested on 15,716 Canadian sera to assess the specificity. These sera were also tested by the buffered plate antigen test. Two ELISA formats were used for assessment of data: the targeting procedure using a positive control serum al...

Chlamydophila psittaci (formerly Chlamydia psittaci) is one of the major pathogens associated with turkey respiratory disease. Devastating outbreaks with high mortality rates, similar to those of 1950 to 1970 in the USA occasionally occur, but respiratory signs without or with low mortality mostly characterize outbreaks now a day. Accurate diagnostic methods should be made available. The presen...

The aims of this study were to evaluate the performance and agreement of various commercial and in-house Neospora caninum antibody assays used in dairy cattle in North America, and to investigate reproducibility of two assays performed in different laboratories. From 1998 to 2005, three enzyme linked immunosorbent assays (ELISAs, a competitive ELISA-VMRD Inc., an indirect ELISA-Biovet Inc., and...

results in this setting, detection rate of the disease is 34.86%, if the high normal titer for the wrights test titer assumed 1/80 and is 44.95% if the titer assumed 1/40, while elisa test had detection rate of 58.72% for those suspicious cases. furthermore, the detection rate for diagnosis of active brucellosis with 2me tests was 17.54% with high normal titer of 1/80 and was 59.64% with a high...

APPLICATION OF ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN RIO GRANDE DO SUL, BRAZIL. The results of an indirect ELISA (I-ELISA) and a competitive ELISA (C-ELISA) for detection of antibody to Brucella abortus in cattle were compared with those of conventional serological tests. The sensitivity of I-ELISA using 230 sera from infected animals was 98.6% and the sp...