Two-hundred and twenty-five strains of Shigella sonnei isolated from sporadic cases of bacillary dysentery were subjected to colicine typing. The result showed that types 15 and 12 were the prevailing types in Hong Kong and two new types which did not fit in with the colicine patterns of the current types were observed. A marked increase in the numbers of strains concurrently resistant to chlor...

the colicine types, biotypes and resistance pattern to antibacterial drugs of 154 strains of shigella sonnei isolated in two different geographical areas of iran, are presented. while a difference in the predominant biotype was observed in the two areas, the resistance pattern and the colicine types were found to be almost identical.

An epidemiological study of Sonne dysentery in Dundee during the years 1971-6 was made by examining, in respect of 1420 isolates of Shigella sonnei, the discriminating power of colicine typing, antibiogram testing, biotyping and resistotyping and the stabilty of the markers they provided. Colicine typing identified nine colicine types, including four not previously described. However, because t...

Colicine V has been obtained from the culture medium in which the colicinogenic bacillus E. coli K357 L_T is grown. The material is electrophoretically homogeneous and proves to be a lipocarbohydrate protein complex identical with the type-specific O antigen of the parent bacillus. Colicine V is toxic both for mice and for rabbits and readily stimulates the elaboration of precipitins and bacter...

Using a freezing-thawing method for extracting colicine from solid media it has been shown that the choice of media for production and diffusion is important. Digest nutrient agar yielded the most colicine and peptone water agar the least. A factor in bacteriological peptone, but absent in a proteose peptone (Difco) and Neopeptone, was responsible for inhibiting production on peptone water agar...

Hemoglobin, its chains, and myoglobin enhance the antibiotic activity of colicine K. These proteins also interact with colicine K and other O antigens to alter their serological activity. The hemoglobin proteins did not alter the serological activities of three Pneumococcus polysaccharides or T4 bacteriophage DNA antigens but did alter the antigenic activity of fetuin. Interaction of hemoglobin...

There can be but little doubt that the bactericidal activity of the colieine K which is elaborated by F~cherichia coli K235 resides in the protein portion of the bacterial somatic antigen (1). In its native state this component is firmly bound to a lipopolysaccharide and the two cannot be separated by the usual chemical or physical techniques. The question has arisen, however, as to what might ...

A temperate bacteriophage was isolated from the colicinogenic strain of Escherichia coli K235 and characterized. This phage, termed PK, is related to P2 virus morphologically, serologically, and, possibly, genetically and it bears no relationship to the T-even phages. It was also demonstrated that PK virus and colicine K differ both in their host range and in their immunological specificity, an...