Abstract Species coculture can increase agro-biodiversity and therefore constitutes an ecological intensification measure for agriculture. Rice-aquatic animal coculture, one type of species has been practiced researched widely. Here, we review recent studies present results a quantitative analysis literature on rice–aquatic systems. We address three questions: (i) rice yield soil fertility be m...

background: spermatogonial stem cells (sscs) are infrequentself-renewing cells among the type a spermatogoniawithin the seminiferous tubules and are the basis of spermatogenesisin mammalian testis. an adequate number of sscs is aprimary requirement for the study of their behavior, regulation, andfurther biomanipulation. objectives: in this paper, we studiedthe development of the primary co-cult...

The present study was designed to test the efficacy, as assessed by blastocyst production, of culturing slaughterhouse-derived bovine oocytes in Ménézo's B2 (B2) medium with or without serum and with or without buffalo rat cell (BRL) coculture. In addition, OPU-derived oocytes were cultured in B2-BRL coculture with or without serum for the first 72 h or TCM 199-BRL coculture with serum and the ...

Standard isolation of human immunodeficiency virus type 1 (HIV-1) from peripheral blood mononuclear cells (PBMC) requires 5 to 20 ml of blood, and the centrifugal separation of PBMC is expensive and time-consuming. Whole-blood coculture techniques use small sample volumes, do not require centrifugation, and allow measurement of the total viral burden in peripheral circulation. We compared the r...

Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have been shown as the most potential stem cell source for articular cartilage repair. In this study, we aimed to develop a method for long-term coculture of human articular chondrocytes (hACs) and hUCB-MSCs at low density in vitro to determine if the low density of hACs could enhance the hUCB-MSC chondrogenic differentiation...

Abstract Immunotherapy success has been limited in treating solid tumors. Bispecific antibody Armed activated T cell (BATs) therapy activates, expands, and arms patient cells ex vivowith bispecific antibodies (BiAb) to produce a “drug” that combines non-MHC restricted cytotoxicity of specificity BiAb. BATs promises improve survival patients with metastatic breast, prostate, pancreatic cancer wi...

background: since embryonic stem (es) cells have the dual ability to proliferate indefinitely and differentiate into multiple tissue types, es cells could potentially provide an unlimited cell supply for human transplantation. objective: in order to study the differentiation of mouse embryonic stem (mes) cells, they were cultured in suspension by using es media without leukemia inhibitory facto...