نتایج جستجو برای: cobas amplicor monitor test

تعداد نتایج: 883872  

Journal: :Journal of clinical microbiology 2002
Evelyn Stelzl Andrea Kormann-Klement Josef Haas Elisabeth Daghofer Brigitte I Santner Egon Marth Harald H Kessler

The COBAS AMPLIPREP instrument for automated sample preparation has recently been introduced. In this study, the COBAS AMPLIPREP/COBAS AMPLICOR HCV MONITOR test, which includes this new molecular device, was evaluated and compared to the COBAS AMPLICOR HCV MONITOR test, which includes a manual extraction protocol. Interassay and intra-assay variation, precision, and linearity were determined, a...

Journal: :modares journal of medical sciences: pathobiology 2007
saeid amel jamehdar farzaneh sabahi mahdi forouzandeh mahboubeh haji abdolbaghi anooshiravan kazemnejad

objective: in this study, a sybr green real-time rt-pcr assay for quantification of hiv-1 viral rna was developed. materials and methods: this assay was performed based on amplification of the pol region of hiv-1 and product analysis by an abi 7500 system. we quantified hiv-1 viral load in 26 seropositive patients by this system and the data were subsequently compared with results obtained wit...

Journal: :Journal of clinical microbiology 2006
Jeffrey J Germer David W Majewski Billy Yung P Shawn Mitchell Joseph D C Yao

The Invader 1.0 assay (Invader HCV Genotyping Assay, version 1.0; Third Wave Technologies, Inc., Madison, WI) has been developed for the rapid differentiation of hepatitis C virus (HCV) genotypes 1 to 6 based on sequence variation within the HCV 5' noncoding (NC) region. In the present study, we evaluated the compatibility of Invader 1.0 with the COBAS MONITOR (COBAS AMPLICOR HCV MONITOR Test, ...

Journal: :Journal of clinical microbiology 2000
G Gerken T Rothaar M G Rumi R Soffredini M Trippler M J Blunk A Butcher S Soviero G Colucci

A clinical evaluation of an automated quantitative PCR assay, the COBAS AMPLICOR HCV MONITOR test, version 2.0 (v2.0), was carried out to assess the performance of this test in comparison with that of the previous, manual version, the AMPLICOR HCV MONITOR test, and with that of nested PCR. Serial dilutions of serum samples infected with genotype 1b, 2a, or 3, as well as synthetic RNA transcript...

Journal: :Journal of clinical microbiology 2004
Angela M Caliendo Jessica Ingersoll Alicia M Green Frederick S Nolte Kirk A Easley

This study compared the sensitivity and viral load values of the AMPLICOR HIV-1 MONITOR microwell version 1.0, microwell version 1.5, and COBAS version 1.5 tests. Based on the percentage of positive replicates, the microwell version 1.5 and COBAS version 1.5 tests are more sensitive than the microwell version 1.0 test. Viral load values obtained with the COBAS version 1.5 test are lower than th...

Journal: :Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2009
Ian M Gould

3. Katsoulidou A, Petrodaskalaki M, Sypsa V, et al. Evaluation of the clinical sensitivity for the quantitation of human immunodeficiency virus type 1 RNA in plasma: comparison of the new Cobas TaqMan HIV-1 with three current HIV-RNA assays–LCx HIV RNA quantitative, Versant HIV-1 RNA 3.0 (bDNA) and Cobas Amplicor HIV-1 Monitor v1.5. J Virol Methods 2006; 131:168–74. 4. Damond F, Roquebert B, Be...

Journal: :Indian journal of medical microbiology 2007
M S Alfaresi A A Elkoush

Hepatitis C virus (HCV) is the major causative agent of non-A and non-B viral hepatitis. Factors associated with disease progression following HCV infection include the viral genotype, the patient's alcohol consumption and viral load. In this study, the COBAS AMPLICOR HCV MONITOR test, a commercially available quantitative assay for HCV RNA, was used for HCV genotyping analysis. Amplification p...

ژورنال: :پژوهش های آسیب شناسی زیستی 0
saeid amel jamehdar department of virology, school of medical sciences, tarbiat modares university, tehran, iran farzaneh sabahi department of virology, school of medical sciences, tarbiat modares university, tehran, iran mahdi forouzandeh department of biotechnology, school of medical sciences, tarbiat modares university, tehran, iran mahboubeh haji abdolbaghi department of infectious diseases, aids research center of iran, faculty of medicine, tehran university of medical sciences, tehran, iran anooshiravan kazemnejad department of biostatistics, school of medical sciences, tarbiat modares university, tehran, iran rosita edalat department of virology, pasteur institute of iran, tehran, iran mehrnaz rasoolinejad

هدف: در این تحقیق روش real time rt-pcrبا استفاده از رنگ سایبرگرین به منظور سنجش کمی ویروس نقص سیستم ایمنی انسانی نوع یک (hiv-1) راه اندازی شد. مواد و روش ها: ایـن آزمایش بر اســاس تــکثیر ناحیه ژن pol ویروس بوده و از دستگاه ترموسایکلرabi 7500 استفاده شد. 26 بیمار مبتلا به hiv-1 با این روش مطالعه شدند و سپس نتایج با آزمون تجارتی استاندارد کوباس آمپلی کور مونیتور تست (cobas amplicor hiv-1 monitor...

Journal: :Journal of clinical microbiology 2005
G I J G Rours R P Verkooyen H F M Willemse E A E van der Zwaan A van Belkum R de Groot H A Verbrugh J M Ossewaarde

The success of large-scale screening for Chlamydia trachomatis depends on the availability of noninvasive samples, low costs, and high-quality testing. To evaluate C. trachomatis testing with pregnant women, first-void urine specimens from 750 consecutive asymptomatic pregnant women from the Rotterdam area (The Netherlands) were collected. Initially, we investigated the performance of three dif...

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