background and objectives: phytoplanktons are organisms with a very high diversities and global distribution in different habitats. the high distribution of phytoplankton is due to ecological flexibility and their ability to tolerate different climatic conditions and environmental stress. phytoplankton is the most sensitive biological indicators of water resources. the purpose of this study was...

the intestinal bacterial diversity of stichopus japonicus was investigated using 16s ribosomal rna gene (rdna) clone library and polymerase chain reaction/denaturing gradient gel electrophoresis (pcr-dgge). the clone library yielded a total of 188 clones, and these were sequenced and classified into 106 operational taxonomic units (otus) with sequence similarity ranging from 88 to 100%. the cov...

The intestinal bacterial diversity of Stichopus japonicus was investigated using 16S ribosomal RNA gene (rDNA) clone library and Polymerase Chain Reaction/Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The clone library yielded a total of 188 clones, and these were sequenced and classified into 106 operational taxonomic units (OTUs) with sequence similarity ranging from 88 to 100%. The cov...

background and objectives: chlorophyceae are important constituents of marine phytoplankton. the taxonomy of chlorophyceae was traditionally based solely on morphological characteristics. in the present research project, genetic diversity was investigated to analyze five species of chlorophyceae from waters of the persian gulf. materials and methods:   a clone library of the ribosomal small sub...

the formation and regulation of vertebrate endogenous intestinal microbiota has been widely studied as the microbiota plays a crucial role in the host nutrition, development, and health. despite the importance of microbiota for host health, it is still unclear whether the endogenous intestinal microorganisms are genetically distinct or whether they are genetically related with each other in dif...

to assess the microbiological status of a biofiltration system used to treat borehole water, filtermatrix samples were analyzed after staining for the presence of active biofilms using confocal laser scanningmicroscopy (clsm). clsm revealed the presence of biofilms on the filter matrix with actively metabolizingmicrobial cells present. thereafter, heterotrophs,manganese oxidizing bacteria (mob)...

To examine the effect of pathogens on the diversity and structure of plant-associated bacterial communities, we carried out a molecular analysis using citrus and huanglongbing as a host-disease model. 16S rRNA gene clone library analysis of citrus roots revealed shifts in microbial diversity in response to pathogen infection. The clone library of the uninfected root samples has a majority of ph...

OBJECTIVE Conventional diagnosis of bacterial vaginosis contains some controversial points. To understand accurately the relationship between clinical stages and the microbiotas, the intravaginal microbial flora was analyzed by the clone library method. STUDY DESIGN Vaginal fluid samples from 31 patients were examined. Lactobacillary grade, Nugent score, culture-based method, and clone librar...

Background: Clone libraries provide researchers with a powerful resource with which to study nucleic acid from diverse sources. Metagenomic clone libraries in particular have aided in studies of microbial biodiversity and function, as well as allowed the mining of novel enzymes for specific functions of interest. These libraries are often constructed by cloning large-inserts (~30 kb) into a cos...