An efficient protocol for the genetic transformation of cell suspension cultures of several citrus cultivars using Agrobacterium is described. Cell suspension cultures of Citrus sinensis ‘Hamlin’, ‘Valencia’ and ‘OLL8’ (an early maturing ‘Valencia’ like somaclone), Citrus unshiu ‘Okitsu wase’ and Citrus reticulata ‘Ponkan’ and ‘W. Murcott’ were transformed using A. tumefaciens EHA105. It was de...

Citrus crops are considered to be relatively poor hosts for Queensland fruit fly, Bactrocera tryoni (Froggatt), as for other tephritid species. Australian citrus growers and crop consultants have reported observable differences in susceptibility of different citrus cultivars under commercial growing conditions. In this study we conducted laboratory tests and field surveys to determine susceptib...

Volatiles from flowers at three blooming stages of nine citrus cultivars were analyzed by headspace-solid phase microextraction (HS-SPME)-GC-MS. Up to 110 volatiles were detected, with 42 tentatively identified from citrus flowers for the first time. Highest amounts of volatiles were present in fully opened flowers of most citrus, except for pomelos. All cultivars were characterized by a high p...

Genetic relationship among 17 citrus commercially important cultivars, including local and some hybrid varieties of Pakistan was evaluated by RAPD (Random Amplified Polymorphic DNA) markers. Fifteen decamer primers were selected out of 25 primers that produced 153 polymorphic bands with 13bands per primer on an average with a total of 188 bands. To analyze the genetic similarity or dissimilarit...

Because most regions of Japan are in the temperate climate zone, many deciduous and evergreen fruit crops are cultivated in Japan. In addition to fruit cultivation in the temperate zone, some southern areas, such as the Ryukyu Islands that stretch from Kyushu to Taiwan, are at the northern limits for tropical fruit production without the need of artificial heating. At present, annual fruit prod...

Bergamottin is a natural furanocoumarin compound with weak polarity. Characterization and quantification of bergamottin were carried out in different fruit tissues of various citrus cultivars. Among the four citrus tissues tested, i.e., flavedo, albedo, segment membrane (SM), and juice sacs (JS) in eight citrus cultivars, the highest bergamottin content was found in the flavedo of Citrus grandi...

Mandarin (Citrus reticulata), citron (Citrus medica), and pummelo (Citrus maxima) are important species of the genus Citrus and parents of the interspecific hybrids that constitute the most familiar commercial varieties of Citrus: sweet orange, sour orange, clementine, lemon, lime, and grapefruit. Citron produces anthocyanins in its young leaves and flowers, as do species in genera closely rela...

The Sudanese citrus industry is mainly based on oldline cultivars of grapefruit, sweet orange and willow leaf mandarin, grafted almost exclusively on sour orange. Infection with graft-transmissible agents, viroids in particular, may have deleterious effects on the productivity of these citrus species and, more importantly, limits the choice of rootstocks should sour orange be replaced by altern...

Huanglongbing (HLB) is currently the most destructive disease of citrus worldwide. Although there is no immune cultivar, field tolerance to HLB within citrus and citrus relatives has been observed at the USDA Picos farm at Ft. Pierce, Florida, where plants have been exposed to a very high level of HLB pressure since 2006. In this study, we used RNA-Seq to evaluate expression differences between...

Florida is the world’s third largest producer of citrus, behind Brazil and China. This 9 billion dollar industry is now under siege by two important diseases citrus greening, also known as huanglongbing (HLB), and citrus canker. Both these diseases are caused by gram negative bacteria. Canker is primarily affecting the grapefruit industry and poses problems with the establishment of new sweet o...