Liquid chromatography is a widely popular technique in clinical laboratories and clinical applications. People evolved and developed various types of chromatography for decades after its initial idea published in 1960s. Mostly people use HPLC-MS, UHPLC techniques to purify antibody, drug isomers and enantiomers. Separating molecules of different sizes by modified HPLC and size exclusion chromat...

For the analysis of residues of veterinary drugs, liquid chromatography (LC) is of increasing importance: some of these molecules are polar, heat-sensitive and/or difRcult to analyse by gas chromatography}mass spectrometry (GC-MS). Moreover, LC is the method of choice for components of high molecular mass. Since the introduction of benchtop LC-MS instruments, there has been an increasing number...

This paper describes potential applications of computer-assisted chemometrics in method development in liquid chromatography. These include modeling of retention (isocratic, gradient, molecular modeling, artificial neural networks), assessment of separation (peak capacity), single and multiple objective optimization approach, advanced optimization algorithms (genetic algorithms, simulated annea...

The measurement of glycosylated haemoglobin in blood is vital for monitoring metabolic control in diabetics [1-3]. The development of many different methods of measurement (based on cation-exchange chromatography, electrophoresis, high performance liquid chromatography [HPLC], affinity chromatography, radio and colorimetric immuno techniques) proves the importance of this assay [4]. Since the f...

The applications of liquid chromatography-mass spectrometry (LC-MS) have exploded in innumerable analytical fields, including pesticide-residue determination. There is no doubt that LC-MS is currently competing with gas chromatography (GC)-MS for the status of reference analytical technique to determine pesticide residues and that its ever-increasing application is bound to the evolution of mod...

Whereas John Hunter (1728-93) the anatomist, zoologist, physiologist, pathologist and museum collector receives unceasing eulogy, his work as a practising surgeon is curiously neglected. As far as can be determined, there are no studies devoted to his instruments and operative technique before a personal investigation in 1991, perhaps for the good reason that these would prove inconclusive. Nev...

Multicomponent analyses were carried out for three types of urinary constituents: steroids, acids, and drugs and drug metabolites. The methods were based on gas-phase analytical techniques, which include the use of instruments and instrumental systems for gas chromatography, gas chromatogra phy-mass spectrometry, and mass spectrometry-com puterization. After isolating an analytical sample, we p...

This review presents the technique of liquid chromatography-mass spectrometry (LC-MS). It brie y outlines how LC-MS works, its different functions, the different types of instruments available, the applications with which it is mainly used and the costs involved. In particular, it highlights the effectiveness of LC-MS in measuring steroids and emphasizes its usefulness in measuring steroids non...