in order to investigate the gas separation ability of a column packed with nanozeolitic material, nano nay zeolite was synthesized and granulated. these uniform granules packed in a chromatographic column were utilized for separation of xe and kr under various operating conditions. with regards to the response peaks obtained from trace injections of xe and kr into the column, the first and seco...

Several methods are available for specifically identifying and assessing the activity of MB isoenzyme of creatine kinase in serum. We compared two electrophoretic techniques with three column-chromatographic ones for efficacy in the diagnosis of myocardial infarction. No technique was considered absolute, but we found surprisingly large errors of diagnosis in the column-chromatographic techniqu...

A high-performance liquid chromatographic method was developed for the analysis of capsaicinoid compounds, the pungent principles of capsicum fruits. A sequential simplex method was applied to optimize the chromatographic response function used to assess the quality of separation by varying the chromatographic parameters. The separation was achieved in 11 min using a C-8 column of 15-cm length ...

P reparative chromatography is the dominant purification technique in the production of biological compounds, especially for bioseparations. The column used is central to the performance of the chromatographic step. To ensure robust and reproducible column performance, columns need to be qualified in an acceptable fashion before each use. Some of the critical elements of column qualification in...

A simple and specific method is presented for the capillary gas chromatographic determination of trazodone in postmortem blood and tissues. The drug and trimethobenzamide, which was used as an internal standard, were extracted with 1% isopropanol in n-butyl chloride. Chromatography was accomplished with a short (4-m) methyl silicone capillary column and hydrogen carrier gas. This combination of...

In this study chromatographic behavior of mouse serum immunoglobulin G (IgG) on a protein G perfusion affinity chromatographic column was investigated experimentally. The results indicate that the protein G column has no non-specific binding to the other proteins in mouse serum but an irreversible adsorption to IgG under the conditions investigated. It was found that variations of the elution s...

Dicaffeoylquinic acids (diCQAs) are plant metabolites and undergo trans-cis-isomerization when exposed to UV irradiation. As such, diCQAs exist in both trans- and cis-configurations and amplify the already complex plant metabolome. However, analytical differentiation of these geometrical isomers using mass spectrometry (MS) approaches has proven to be extremely challenging. Exploring the chroma...