نتایج جستجو برای: chromatin condensation
تعداد نتایج: 75031 فیلتر نتایج به سال:
background: current microscopic experimental methods cannot diagnose dna damages present in spermatozoa .therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. as reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. also, chromomycin a3 is used for evaluation of the ...
Background and Aim: Sperm cryopreservation is a common technique used for management of male infertility. But this method has detrimental effects on sperm DNA and chromatin quality. Evaluation of different markers associated with the health of genetic material of sperm is beneficial for determination of the fertility of sperm. The aim of this study was to assess the effect of sperm chromatin co...
Although chromatin condensation is one of the hallmarks of apoptosis, its relationship with DNA fragmentation has been controversial. We show here that apoptotic chromatin condensation is regulated by nucleoplasmin, a protein that decondenses sperm chromatin during male pronuclear assembly. In Xenopus egg extracts, nucleoplasmin is tyrosine-dephosphorylated during apoptosis. This dephosphorylat...
سرطان مثانه رشد غیر طبیعی و خارج از کنترل سلول های مثانه است که خارج از مکانیسم های معمول کنترل بدن اتفاق می افتد. سرطان مثانه رایج ترین تومور سیستم ادراری می باشد. استفاده از شیمی درمانی یکی از راهکار های اصلی برای کاهش دادن سرعت پیشرفت سرطان و یا درمان آن می باشد. تعــداد زیادی از فاکتور های شیمی درمانی به عنوان عوامل دارای سمیت سلولی طبقه بندی می شوند که دارای اثرات مشابه بر روی سلول های نرم...
Apaf-1(-/-) or caspase-3(-/-) cells treated with a variety of apoptosis inducers manifest apoptosis-associated alterations including the translocation of apoptosis-inducing factor (AIF) from mitochondria to nuclei, large scale DNA fragmentation, and initial chromatin condensation (stage I). However, when compared with normal control cells, Apaf-1(-/-) or caspase-3(-/-) cells fail to exhibit oli...
Akt promotes cell survival by phosphorylating and inhibiting components of the intrinsic cell death machinery. Akt translocates into the nucleus upon exposure of cells to survival factors, but little is known about its functions in the nucleus. Here, we show that acinus, a nuclear factor required for apoptotic chromatin condensation, is a direct target of Akt. We demonstrate that Akt phosphoryl...
Hallmarks of the terminal stages of apoptosis are genomic DNA fragmentation and chromatin condensation. Here, we have studied the mechanism of condensation both in vitro and in vivo. We found that DNA fragmentation per se of isolated nuclei from non-apoptotic cells induced chromatin condensation that closely resembles the morphology seen in apoptotic cells, independent of ATP utilization, at ph...
Apoptosis is the predominant form of cell death observed in a variety of physiological and pathological conditions such as cancer involution, insect metamorphosis, the development of the immune and nervous systems, and embryogenesis. The typical nuclear changes taking place in apoptotic cells include extensive condensation of chromatin and internucleosomal DNA fragmentation into units of 200 ba...
OBJECTIVE This study was performed to evaluate testicular sperm chromatin condensation using aniline blue-eosin (AB-E) staining and its effects on IVF-ET. METHODS Chromatin condensation was analyzed using AB-E staining in 27 cases of testicular sperm extraction. There were 19 cases of obstructive azoospermia (OA) and 8 cases of non-obstructive azoospermia (NOA) in IVF-ET. Mature sperm heads w...
We report the development of a new method for producing mitotic extracts from tissue culture cells. These extracts reproducibly promote the condensation of chromatin in vitro when incubated with purified interphase nuclei. This condensation reaction is not species specific, since nuclei from chicken, human, and hamster cell lines all undergo chromatin condensation upon incubation with the extra...
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