objective(s): this paper aims to investigate synergistic inhibition of polysaccharide from sepia esculenta ink (sip), a newly isolated marine polysaccharide in our laboratory, on breast cancer mda-mb-231 cells exposed to cisplatin. materials and methods: cell viability of mda-mb-231 cells was determined by cck 8 assay. median-effect concentration was analyzed using chou-talalay method that was ...

This brief perspective article focuses on the most common errors and pitfalls, as well as the do's and don'ts in drug combination studies, in terms of experimental design, data acquisition, data interpretation, and computerized simulation. The Chou-Talalay method for drug combination is based on the median-effect equation, derived from the mass-action law principle, which is the unified theory ...

This brief article focuses on two aims: i) To investigate the in vitro pharmaco-dynamic interactions of combining synthetic potent microtubule targeting anticancer agent, Fludelone (FD) with cyto-protective agent, Panaxytriol (PXT) derived from Panax ginseng, and ii) To illustrate step-by-step operation for conducting two-drug combination in vitro using the combination index method, in terms of...

This study investigates the possible synergistic stimulatory effects of prebiotic chitooligosaccharide and some commercial prebiotics on probiotic growth. Different combination with inulin, fructooligosaccharide, lactulose were prepared their ability to stimulate growth strains was evaluated. Following Chou-Talalay method, index calculated used for assessment. The data showed that most combinat...

Advances in the theory of combination therapy (1) and development of new targeted drugs are leading to a new emphasis on drug combination experiments. Many studies continue to use the median-effect method of Chou–Talalay (2). However, the median-effect method follows a similar logic to the defunct Scatchard analysis in pharmacology, which has been replaced by nonlinear modeling. Like Scatchard ...

We describe a novel improved approach to the synthesis of the important and well-known heat shock protein 70 inhibitor (HSP70), pifithrin-μ, with corresponding and previously unreported characterisation. The first example of a combination study comprising HSP70 inhibitor pifithrin-μ and cisplatin or oxaliplatin is reported. We have determined, using the Chou-Talalay method, (i) moderate synergi...

Materials and methods CWR22Rv1 cells (a castrate-resistant human PCa cell line) were treated with a polyherbal mixture of botanicals (PHBs) consisting of 10 herbal extracts [1] and we investigated whether inhibition of cell proliferation was linked to the activity of 5’adenosine monophosphate (AMP)activated protein kinase (AMPK), a potential tumor suppressor protein that functions as a central ...

Glioblastoma is the most malignant brain tumor and presents high resistance to chemotherapy radiotherapy. Surgery, radiotherapy with temozolomide are only treatments against this tumor. New targeted therapies, including epigenetic modulators such as 3‑deazaneplanocin A (DZ‑Nep; an EZH2 inhibitor) panobinostat (a histone deacetylase being tested in vitro, together temozolomide. The present study...