In the present study, a simple and efficient extraction method based on dispersive liquid-liquid microextraction prior to UV-Vis spectrophotometry was developed for the preconcentration and determination of copper ions in environmental samples. Briefly, cupric ions (Cu II) were reduced to cuprous (Cu I) with addition of hydroxyl amine hydrochloride and formed hydrophobic chelates with neocuproi...

AIM To time the onset of cytomegalovirus (CMV) infection in patients (n = 39) with CMV associated neonatal cholestasis by analysing CMV DNA on Guthrie cards sampled at 3 days of age. METHODS CMV infection was diagnosed by serology/urine isolation or by CMV DNA detection (polymerase chain reaction) in liver biopsy specimens. In order to time the infection dry blood filter paper discs were punc...

we have evaluated the nmr shielding tensors for a:t,g:c,a:u in watson-crick, and u-quartet. we have computed nmr shielding tensors at b31yp level by using 6-31g(d) basis set. we have compute anisotropy and asymmetry in a:t,g:c,a:u and u-quartet. the nmr shielding tensors were calculated using the giao method. the natural bonding orbital analysis (nbo) were performed. nbo calculation have been ...

The antibacterial activity of various solvent fractions of Balaguduchi, Dhanadanayanadi and Dasamoolabala was studied against the isolated nosocomial pathogens by in vitro method. The methanol, acetone, choloroform, acetone fractions of test drugs and decoctions exhibited different levels of antibacterial activity from low to very high level against the gram positive and gram -negative bacteria...

Sir,?I have recently returned to a well-known firm of druggists in Rangoon a consignment of chloroform which was labelled chloloform pure B. P. (made from pure alcohol dbc." It appeared to take an excessive amount to ansesthetise : patients became very cyanotic with irregular breathing: the pulse was often weak : and many had much vomiting, and felt very ill for two days. A sample was sent to t...

The analysis of corticosteroids (CDS) was preliminarily investigated using a DU PONT 830 High Pressure Liquid Chromatography (HPLC) equipped with a UV detector and a Permaphase column ETH. 1) Firstly, the best condition for the separation of mixed CS was investigated. In a linear gradient elution, ten authentic steroids, varying greatly in polarity (including native CS such as DOC, Corticostero...

1. The transfer of mannose from GDP-(U-14-C)mannose into endogenous acceptors of bovine adrenal medullla and rat parotid was studied. The rapidly labelled product, a glycolipid, was partially purified and characterized. 2. It was stable to mild alkaline hydrolysis but yielded (14-C)mannose on mild acid hydrolysis. It co-chromatographed with mannosyl phosphoryl dolichol in four t.l.c. systems an...

Natural products from fungi are reported for various anticancer, antioxidant and other biological activities. So in search for novel bioactives from fungi isolated and identified from infected leaves of Rhododendron sp. Thus the isolated fungus was identified as Monochaetia kansensis, was extracted with variety of solvents of increasing polarity such as chloroform, Dichloromethane and ethylacet...

SIMPLE, RAPID TEST-TUBE EXIRACTION TECHNIC for the determination of urinary 17-ketosteroids has seen developed in our laboratories. A complete urinary 17-ketosteroid determination was carried out in a glass stoppered photometer cuvet by combining this extraction technic with a colorimetric method. Three colorimetric methods, Zimmermann (1), (2), Pincus (3), and Cohen and Kaluszyner (4) were com...