We have developed a succinyldicholine-based assay for serum cholinesterase (EC 3.1.1.8) to help establish whether patients with suspected sensitivity to drugs of this type have enzyme abnormalities that cannot be detected by conventional laboratory techniques. Although the method discriminates between cholinesterase activities of drug-sensitive and nonsensitive people as well as an assay involv...

Previous investigators (Frawley, Hagan & Fitzhugh, 1952; Davison, 1955; Kewitz & Nachmansohn, 1957) have observed that the rate of recovery in vivo of inhibited brain true cholinesterase depended on which inhibitor was used. With diiwopropyl phosphorofluoridate (Frawley et al. 1952; Davison, 1955; Kewitz & Nachmansobn, 1957) recovery was slow, whereas with tetraethyl pyrophosphate (Davison, 195...

The genus Sarcococca is widely distributed in South-East Asia and it comprises 14 species. The genus is traditionally used for gastrointestinal ulcers, infections, pain and in rheumatic fevers. Recently, our group has derived a comprehensive SAR relationship picture for a new series of natural cholinesterase inhibitors isolated from Sarcococca saligna (syn. S. pruniformis, Buxaceae). The fracti...

biomarkers are assigned as early warning systems regarding aquatic organisms exposed to pollutants. among which, however, measurement of esterase enzymes activity specially cholinesterase of fish tissues, is a marker for exposure to organophosphate pesticide and carbamates, and this biomarker has been used widely in broad range of investigations. a number of capoeta capoeta gracilis (:cyprinida...

The genus Sarcococca is widely distributed in South-East Asia and it comprises 14 species. The genus is traditionally used for gastrointestinal ulcers, infections, pain and in rheumatic fevers. Recently, our group has derived a comprehensive SAR relationship picture for a new series of natural cholinesterase inhibitors isolated from Sarcococca saligna (syn. S. pruniformis, Buxaceae). The fracti...

Human cholinesterase exists in two forms--acetylcholinesterase located in tissue microsomes and red blood cells and serum cholinesterase found in serum or plasma. The two enzymes display marked differences in structure, substrate specificity, biological function, and origin. Contemporary methods employ acylthiocholine as substrate for serum cholinesterase and a second coupled reaction of thioch...

Molecular docking is a promising and reliable technology for the purpose of discovering lead compounds via virtual screening. In addition to allowing testing large number compounds, it also allows determination how selected inhibit targeted protein/receptor based on scoring function ranking. Because selective cholinesterase monoamine oxidase inhibitors play critical role in treatment Alzheimer'...

The cholinesterase activity of the sera of 25 subjects diagnosed as homozygotes for the silent cholinesterase gene was studied by a sensitive enzymatic method employing several thiocholine esters and various inhibitors, and by disc electrophoretic, immunochemical, and chromatographic methods.(a) With one exception, the sera fell into two classes by all criteria. One class (type I, 16 cases) had...