background: cholesterol oxidase (cho) has various clinical and industrial applications. recently, microbial cho have received a great attention for their wide usage in medicine. here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for cho production and biochemical characterizations of produced cho enzyme were described. finally, cho gene was cloned int...

Thirteen species of Streptomycetes, Arthrobacters and Mycobacterium were studied for the determination of a potential cholesterol oxidase producer. Only Streptomyces fradiae and Streptomyces rishiriences were found to be capable of producing this enzyme. Streptomyces fradiae was selected for the production of cholesterol oxidase and cultured in different media to find optimum media and con...

Oxygen free radicals have the ability to oxidize cholesterol. However, nothing is known about the effects of cholesterol oxidation on ion transport in isolated myocardial membranes. The purpose of the present study was to investigate the effects of in situ oxidative modification of sarcolemmal cholesterol on Ca2+ flux. Cholesterol oxidase was used to oxidatively modify membrane cholesterol. Aft...

The membrane of vesicular stomatitis virus could be readily enriched with exogenous cholesterol by exposing virion suspensions to serum lipoproteins forming complexes with cholesterol or to a carrier of polyvinylpyrrolidone/bovine serum albumin mixed with cholesterol. These procedures increased the cholesterol content of the virion membrane f rom -35 to -60% of the total lipid. Endogenous chole...

The distribution of cellular unesterified cholesterol was studied in fibroblasts, which had been depleted of plasma membrane sphingomyelin by exposure to exogenous sphingomyelinase. This treatment has previously been shown to induce an increase in cholesterol esterification, a decrease in the biosynthesis of cholesterol, and a decreased susceptibility of cell cholesterol to oxidation with chole...

Background: Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned int...

Cholesterol oxidase represents a novel type of insecticidal protein with potent activity against the cotton boll weevil (Anthonomus grandis grandis Boheman). We transformed tobacco (Nicotiana tabacum) plants with the cholesterol oxidase choM gene and expressed cytosolic and chloroplast-targeted versions of the ChoM protein. Transgenic leaf tissues expressing cholesterol oxidase exerted insectic...