Chloroplast RNA is synthesized in dark-grown radish cotyledons at about one-third the rate of that in the light. The synthesis, however, continues for longer in the dark and the percentage of chloroplast RNA can approach that in light-grown tissue. Light stimulates the synthesis and accumulation of both cytoplasmic and chloroplast RNA, but shows a 4-fold greater stimulation of the chloroplast R...

Abstract Members of the pentatricopeptide repeat (PPR) protein family act as specificity factors in C-to-U RNA editing. The expansion PPR superfamily plants provides sequence variation required for design consensus-based RNA-binding proteins. We used this approach to a synthetic editing factor target one sites Arabidopsis chloroplast transcriptome recognised by natural CHLOROPLAST BIOGENESIS 19...

In a companion study on the site of synthesis of chloroplast and mitochondrial RNA (7), it was shown that when dividing greening cells of Ochromonas danica were fed a radioactive RNA precursor, both the chloroplast and mitochondria as well as the nucleus were heavily labeled after a short labeling time, whereas a lag was observed in the labeling of the cytoplasmic matrix. These data were interp...

Methods are described which provide good recoveries of non-degraded chloroplast and non-chloroplast RNAs from Euglena gracilis var. bacillaris. These have been characterized by comparing the RNA from W(3)BUL (an aplastidic mutant of Euglena), with that of wild-type cells which have been resolved into chloroplast and non-chloroplast fractions. Using E. coli RNA as a standard, the RNAs from W(3)B...

The effect of rifampin, an inhibitor of bacterial DNA-dependent RNA polymerase, was studied in Chlamydomonas reinhardi. It was shown, in vivo and in vitro, that chloroplast-located, but not nuclear, DNA-dependent RNA polymerase is inhibited by this drug. The inhibition of chloroplast RNA polymerase results in the inhibition of chloroplast rRNA synthesis, and thus in the loss of chloroplast ribo...

Plastid genes are transcribed by DNA-dependent RNA polymerase(s), which have been incompletely characterized and have been examined in a limited number of species. Plastid genomes contain rpoA, rpol, rpoC1, and rpoC2 coding for a, 8, B', and 8" RNA polymerase subunits that are homologous to the a, 8, and 8' subunits that constitute the core moiety of RNA polymerase in bacteria. However, genes w...

A species of RNA that migrates on 10% (w/v) polyacrylamide gels between 5S and 4S RNA was detected in spinach chloroplasts. This RNA (referred to as 4.5 S RNA) was present in amounts equimolar to the 5S RNA and its molecular weight was estimated to be approx. 33 000. Fractionation of the chloroplast components showed that the 4.5S RNA was associated with the 50 S ribosomal subunit and that it c...

Background: Gossypium thurberi is a wild diploid species that has been used to improve cultivated allotetraploid cotton. G. thurberi belongs to D genome, which is an important wild bio-source for the cotton breeding and genetic research. To a certain degree, chloroplast DNA sequence information are a versatile tool for species identification and phylogenetic implications in plants. Different ch...

In the unicellular green alga Chlamydomonas reinhardtii, the chloroplast-encoded tscA RNA is part of a tripartite group IIB intron, which is involved in trans-splicing of precursor mRNAs. We have used the yeast three-hybrid system to identify chloroplast group II intron RNA-binding proteins, capable of interacting with the tscA RNA. Of 14 candidate cDNAs, 13 encode identical polypeptides with s...